Experimental research on RNA interference targeting Livin in treatment of gastric carcinoma in nude mice

QIAO Min; et al

doi:10.13406/j.cnki.cyxb.2010.02.034

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Experimental research on RNA interference targeting Livin in treatment of gastric carcinoma in nude mice

更新时间：2024-01-17

- Experimental research on RNA interference targeting Livin in treatment of gastric carcinoma in nude mice
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 196-198(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院消化内科
  2. 重庆医科大学附属第一医院实验研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.034
  CLC： R73-3
- Published：2010，
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[1]谯敏,向廷秀,王丕龙.RNA干扰抑制裸鼠皮下移植瘤中Livin基因表达的实验研究[J].重庆医科大学学报,2010,35(02):196-198.

QIAO Min, et al. Experimental research on RNA interference targeting Livin in treatment of gastric carcinoma in nude mice. [J]. Journal of Chongqing Medical University 35(2):196-198(2010)
[1]谯敏,向廷秀,王丕龙.RNA干扰抑制裸鼠皮下移植瘤中Livin基因表达的实验研究[J].重庆医科大学学报,2010,35(02):196-198. DOI： 10.13406/j.cnki.cyxb.2010.02.034.

QIAO Min, et al. Experimental research on RNA interference targeting Livin in treatment of gastric carcinoma in nude mice. [J]. Journal of Chongqing Medical University 35(2):196-198(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.034.

摘要

目的:利用RNA干扰（RNA interference

RNAi）技术在裸鼠皮下移植瘤中抑制Livin基因表达

探讨RNA干扰用于胃癌治疗的可行性。方法:SGC-7901细胞注射人裸鼠皮下

建立裸鼠皮下移植瘤的模型

设计靶向Livin基因的小干扰RNA（Small interference RNA

siRNA）

构建重组表达质粒pTZU6+1-siRNA-Livin

并导入裸鼠皮下移植瘤中

在体内诱导RNAi

采用RT-PCR和免疫化学检测Livin基因表达

Western blot检测Bax和Bcl-2改变。结果:重组质粒pTZU6+1-siRNA-Livin导入裸鼠皮下移植瘤后14d瘤体体积明显减小

Livin的mRNA和蛋白表达均明显下调。结论:RNA干扰明显抑制了靶基因Livin的表达及肿瘤细胞生长

可能与上调Bax的表达、下调Bcl-2

从而诱导肿瘤细胞凋亡有关。

Abstract

Objective:To investigate the feasibility of gastric carcinoma gene therapy by utilizing RNA interference(RNAi)to inhibit the expression of Livin in vivo.Methods:SGC-7901was injected into nude mice to form hypodermical gastric carcinoma.Small interference RNA(siRNA)homologous to Livin gene were designed

pTZU6+ 1-siRNA-Livin vector was constructed and injected into tumor to induce RNAi.The changes of Livin gene expression were detected with RT-PCR and immunochemistry

the expression of Bax and Bcl-2 were detected by western blot.Results:14 days after transfected with pTZU6+l-siRNA-Livin

both the mRNA and the protein of Livin decreased obviously.The level of Bax was up-regulated and the level of Bcl-2 was decreased.Conclusion:RNA interference targeting Livin inhibits Livin expression and tumor growth

which may be related to up-regulation of Bax and down-regulation of Bcl-2.

关键词

RNA干扰胃癌livin

Keywords

RNA interferencegastric carcinomalivin

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