Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H<sub>22</sub>)

YANG Lin; et al

doi:10.13406/j.cnki.cyxb.2010.02.033

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Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H₂₂)

更新时间：2024-01-17

- Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H₂₂)
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 192-195(2010)
- 作者机构：
  
  重庆医科大学基础医学院病理生理教研室、干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.033
  CLC： R73-3
- Published：2010，
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[1]杨麟,沈宜,汪少华,李静,汤为学,郑维萍.热休克小鼠肝癌细胞(H_(22))源Exosomes的体内抗肝癌作用研究[J].重庆医科大学学报,2010,35(02):192-195.

YANG Lin, et al. Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H₂₂). [J]. Journal of Chongqing Medical University 35(2):192-195(2010)
[1]杨麟,沈宜,汪少华,李静,汤为学,郑维萍.热休克小鼠肝癌细胞(H_(22))源Exosomes的体内抗肝癌作用研究[J].重庆医科大学学报,2010,35(02):192-195. DOI： 10.13406/j.cnki.cyxb.2010.02.033.

YANG Lin, et al. Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H₂₂). [J]. Journal of Chongqing Medical University 35(2):192-195(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.033.

摘要

目的:在荷瘤BALB/c小鼠观察热休克小鼠肝癌细胞(H

)源Exosomes（HS-Exo）激发宿主抗肝癌免疫应答的效应。方法:用自行分离纯化的小鼠肝癌细胞H

源Exosomes及HS-Exo免疫小鼠

然后给小鼠皮下接种H

肿瘤细胞

观察肿瘤的生长状况。用MTT法检测小鼠脾细胞增殖情况和细胞毒活性;用免疫组织化学法检测肿瘤组织中CD

、CD

T淋巴细胞浸润情况。BALB/c小鼠皮下接种H

细胞

肿瘤长至可触及大小后

分别注射Exosomes及HS-Exo进行治疗

观察各组肿瘤大小及其小鼠存活期。结果:与对照组Exosomes相比

HS-Exo能使肿瘤出现时间延迟

肿瘤生长缓慢（P

0.05）;更能促进脾淋巴细胞增殖并增强其细胞毒活性（P

0.05）

能更有效的诱导CD

和CD

T细胞活化（P

0.05）;更为显著的抑制了小鼠的肿瘤生长（P

0.05）。结论:HS-Exo具有更强的免疫活性

能更好的诱导小鼠抗H22肝癌作用

有针对亲本细胞的免疫保护作用

并且具有更为显著的肿瘤治疗作用。

Abstract

Objective:To investigate the induction of antitumor response and its immune mechanism by H

cell-derived heat shocked Exosomes（HS-Exo）.Methods:The mice were immunized by H

cell-derived Exosomes and HS-Exo

then inoculated with the wild type H

and finally the tumor growth were observed.Lymphocyte proliferation and specific cytotoxic activity of mice splenic cells was determined by MTT.Infiltration of lymphocytes in tumor tissues was analyzed by immuno-histochemical staining of CD

.Results: The tumor size was smaller in HS-Exo group（P

0.05）.HS-Exo could induce more lymphocyte proliferation（P

0.05）.HS-Exo could generate stronger specific cytotoxic spleen lymphocyte response.Obviously increased infiltrations of CD

T cells were observed in the tumor sites of HS-Exo group（P

0.05）.And HS-Exo was more effective to restrain tumor growth（P

0.05）.Conclusion:Vaccination of H

cell-derived HS-Exo is more effective in the induction of protective immunity and could exert better effect on H

to treat the experimental tumor.

关键词

Exosomes热休克肝细胞癌肿瘤疫苗

Keywords

Exosomesheat shockhepatocellular carcinomatumor vaccine

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Related Institution

重庆医科大学基础医学院病理生理学教研室

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Department of Biochemistry and Molecular Biology，School of Basic Medicine，Chongqing Medical University

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Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H22)

DOI：10.13406/j.cnki.cyxb.2010.02.033

摘要

Abstract

关键词

Keywords

references

Antitumor effects of the Exosomes derived from heat shocked mouse hepatoma carcinoma cell line(H₂₂)