The reparation of metallothionein to the injuried neuron after cerebral ischemia and reperfusion in rats

LI Zheng; et al

doi:10.13406/j.cnki.cyxb.2010.02.028

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The reparation of metallothionein to the injuried neuron after cerebral ischemia and reperfusion in rats

更新时间：2024-01-17

- The reparation of metallothionein to the injuried neuron after cerebral ischemia and reperfusion in rats
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 202-205(2010)
- 作者机构：
  
  重庆医科大学药学院药理教研室,重庆市生物化学与分子药理学重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.028
  CLC： R743.3
- Published：2010，
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[1]李峥,董志.金属硫蛋白对全脑缺血再灌注后损伤神经的修复作用[J].重庆医科大学学报,2010,35(02):202-205.

LI Zheng, et al. The reparation of metallothionein to the injuried neuron after cerebral ischemia and reperfusion in rats. [J]. Journal of Chongqing Medical University 35(2):202-205(2010)
[1]李峥,董志.金属硫蛋白对全脑缺血再灌注后损伤神经的修复作用[J].重庆医科大学学报,2010,35(02):202-205. DOI： 10.13406/j.cnki.cyxb.2010.02.028.

LI Zheng, et al. The reparation of metallothionein to the injuried neuron after cerebral ischemia and reperfusion in rats. [J]. Journal of Chongqing Medical University 35(2):202-205(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.028.

摘要

目的:观察金属硫蛋白（Metallothionein

MT）外源性补充对大鼠全脑缺血再灌注（Cerebral ischemia and reperfusion

I/R）损伤的保护作用

并初步探讨其可能机制。方法:建立I/R模型

采用低血压并双侧颈总动脉夹闭的全脑缺血再灌注模型。缺血20 min再灌注1 d后

测行为学指标

石蜡切片HE染色观察海马神经元形态变化

并用免疫组化方法检测海马神经生长因子-β（Nerve growth factor-β

NGF-β）的表达。结果:MT脑室注射给药（1.4、4.2 mg/kg）能剂量依赖性的减轻大鼠行为学症状

增加大鼠海马神经元NGF-β的表达（0.46、1.4、4.2 mg/kg）。同时病理组织学观察还发现

MT（0.46、1.4、4.2 mg/kg）能显著减少I/R大鼠海马区神经元的固缩

增加存活细胞的数量

减轻脑损伤。结论:MT对脑缺血再灌注损伤有神经保护作用

可能机制为促损伤神经元修复。

Abstract

Objective:To observe the protect action of exogenous metallothionein on rats’ cerebral ischemia and reperfusion and explore its possible mechanism.Methods:I/R modle was established.After 20 min ischemia and 1 d reperfusion

determined the ethology index and the HE staining was determined to see the morphologic changes of neurons in hippocampus.The expression of NGF-βin hippocampus was determined by immunohistochemistry. Resluts:Metallothionein cerebral ventricle injection(1.4、4.2 mg/kg)could significantly reduce the ethology symptom by dose dependent

the expression of NGF-βin hippocampus also was increased(0.46、1.4、4.2 mg/kg).Meanwhile

the pathological examination showed that the karyopycnosis of neurons in hippocampus with I/R in rats could be decreased by MT uncommonly

which mitigated the injury of brain. Conclusion:MT can protect the brain from I/R injury

its active mechanism may be related to repair impaired neurones.

关键词

金属硫蛋白神经生长因子脑缺血再灌注

Keywords

Metallothioneinnerve growth factorCerebral ischemia and reperfusion

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