Effects of valsartan on the expression of MMP-2 and MMP-9 in the brain tissue of renal hypertensive rats after cerebral ischemia-reperfusion

ZHU Hong; et al

doi:10.13406/j.cnki.cyxb.2010.02.027

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Effects of valsartan on the expression of MMP-2 and MMP-9 in the brain tissue of renal hypertensive rats after cerebral ischemia-reperfusion

更新时间：2024-01-17

- Effects of valsartan on the expression of MMP-2 and MMP-9 in the brain tissue of renal hypertensive rats after cerebral ischemia-reperfusion
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 183-186(2010)
- 作者机构：
  
  遵义医学院附属医院神经内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.027
  CLC： R741;R544.1
- Published：2010，
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[1]祝红,詹剑,杨丽.缬沙坦对肾性高血压大鼠脑缺血再灌注后脑组织MMP-2和MMP-9表达的影响[J].重庆医科大学学报,2010,35(02):183-186.

ZHU Hong, et al. Effects of valsartan on the expression of MMP-2 and MMP-9 in the brain tissue of renal hypertensive rats after cerebral ischemia-reperfusion. [J]. Journal of Chongqing Medical University 35(2):183-186(2010)
[1]祝红,詹剑,杨丽.缬沙坦对肾性高血压大鼠脑缺血再灌注后脑组织MMP-2和MMP-9表达的影响[J].重庆医科大学学报,2010,35(02):183-186. DOI： 10.13406/j.cnki.cyxb.2010.02.027.

ZHU Hong, et al. Effects of valsartan on the expression of MMP-2 and MMP-9 in the brain tissue of renal hypertensive rats after cerebral ischemia-reperfusion. [J]. Journal of Chongqing Medical University 35(2):183-186(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.027.

摘要

目的:通过观察血管紧张素Ⅱ受体拮抗剂（Angiotensin receptor blocker

ARB）对肾性高血压大鼠脑缺血再灌注后脑组织中基质金属蛋白酶-2（Matrix metalloproteinase-2

MMP-2）和基质金属蛋白酶-9（Matrix metalloproteinase-9

MMP-9）表达的影响

探讨ARB的脑保护机制。方法:将Wistar雄性大鼠32只随机分为高血压组和正常血压组。前组采用狭窄肾动脉方法建立肾性高血压大鼠模型

再随机分为高血压缺血再灌注组（Hypertension ischemic reperfusion

HIR）和HIR缬沙坦组（G）

分别采用生理盐水和缬沙坦12mg/kg进行灌胃治疗4周;正常血压组分为假手术组（N）和缺血再灌注组（Ischemic reperfusion

IR）

分别行假手术和缺血再灌注处理。采用大脑中动脉线栓法造成大脑缺血再灌注模型

缺血时间为2 h

再灌注22 h。采用免疫组织化学技术检测脑组织MMP-2和MMP-9的表达

用图象分析仪测定灰度值。结果:与N组比较

IR组MMP-2、MMP-9灰度值明显增高（P<0.01）;与IR组比较

HIR组MMP-2、MMP-9灰度值增高（P<0.05）;与HIR组比较

G组MMP-2、MMP-9灰度值减少（P<0.01）。结论:高血压加重缺血再灌注后脑组织MMP-2和MMP-9的表达;ARB能明显抑制脑组织MMP-2和MMP-9的表达。

Abstract

Objective:To explore the mechanisms of angiotensinⅡreceptor antagonist(ARB)-induced brain protection by investigating the effect of ARB on the expression of metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)in the brain of renal hypertensive rats after cerebral ischemia-reperfusion.Methods:Thirty-two male Wistar rats were randomly divided into a hypertensive group and a normal blood pressure group.Hypertension was produced by renal artery occlusion.The rats in the renal hypertensive group were randomly sub-divided into an ischemia-reperfusion(hypertension ischemic reperfusion HIR)group treated with normal saline and a HIR valsartan(G)group treated with valsartan(12 mg/kg per day)by gavage for 4 weeks.The normal blood pressure group was sub-divided into a sham operated group(N)and ischemia-repeffusion group(IR).Cerebral ischemia was induced by middle cerebral artery occlusion with suture for 2 hours followed by 22 hours of reperfusion.The expression of MMP-2 and MMP-9 in brain tissue was detected by immunohistochemistry and their gray values were measured using image analysis.Results:The gray values of MMP-2 and MMP-9 in the IR group were significantly higher than those in the N group(P<0.01).The gray values of MMP-2 and MMP-9 in the HIR group were significantly increased when compared with that in the IR group(P<0.05).The gray values of MMP-2 and MMP-9 in the G group were reduced when compared with that in the HIR group(P<0.01).Conclusion:Hypertension aggravates ischemia- reperfusion-induced expression of MMP-2 and MMP-9 in brain tissue.The increased expression of MMP-2 and MMP-9 in brain tissue can be inhibited by angiotensinⅡreceptor antagonist.

关键词

缬沙坦肾性高血压缺血再灌注损伤基质金属蛋白酶-2基质金属蛋白酶-9

Keywords

valsartanRenal hypertensionischemia-reperfusion injurymatrix metalloproteinase-2matrix metalloproteinase-9

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