Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate

LI Long-hui; et al

doi:10.13406/j.cnki.cyxb.2010.02.015

您当前的位置：

首页 >

文章列表页 >

Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate

更新时间：2024-01-17

- Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 179-182(2010)
- 作者机构：
  
  1. 重庆医科大学附属第二医院消化内科
  2. 重庆医科大学干细胞和组织工程研究室病理生理学教研室
  3. 重庆医药高等专科学校基础部
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.015
  CLC： R575.5
- Published：2010，
扫描看全文
[1]李龙辉,李龙江,汤为学,左国庆,廖于.甘利欣作用前后体外酒精性脂肪肝细胞模型甘油三酯水平变化及其可能作用机制[J].重庆医科大学学报,2010,35(02):179-182.

LI Long-hui, et al. Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate. [J]. Journal of Chongqing Medical University 35(2):179-182(2010)
[1]李龙辉,李龙江,汤为学,左国庆,廖于.甘利欣作用前后体外酒精性脂肪肝细胞模型甘油三酯水平变化及其可能作用机制[J].重庆医科大学学报,2010,35(02):179-182. DOI： 10.13406/j.cnki.cyxb.2010.02.015.

LI Long-hui, et al. Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate. [J]. Journal of Chongqing Medical University 35(2):179-182(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.015.

摘要

目的:研究甘利欣（Diammonium glycyrrhizinate

DIG）作用前后

体外诱导的酒精性脂肪肝（Alcoholic fatty liver

AFL）细胞模型甘油三酯（Triglyceride

TG）水平变化及其可能的作用机制。方法:MTT法筛选最佳作用浓度

透射电镜观察细胞超微结构改变

全自动生化仪检测细胞内甘油三酯水平

免疫细胞化学（Immunocyto-chemistry

ICC）方法观察PPARγ、SREBP-1、SCAP的表达。结果:（1）MTT法筛选出2.273μg/ml为甘利欣最佳作用浓度。（2）透射电镜观察Ld30细胞发现胞浆内存在大量脂滴（3）全自动生化仪测得Ld30细胞内甘油三酯浓度为（1.84±0.16）mmol/L

甘利欣处理组为（1.05±0.12）mmol/L

差异有统计学意义（P<0.01）。（4）与Ld30组相比较

甘利欣处理组PPARγ、SREBP-1、SCAP表达明显减弱

差异有统计学意义（P<0.01）。结论:甘利欣有减轻体外诱导的酒精性脂肪肝细胞脂肪变的作用

其作用机制可能是通过下调PPARγ、SREBP-1、SCAP表达

从而抑制了细胞内脂肪生成。

Abstract

Objective:To study the triglyceride level changes with or without Diammounium Glycyrrhizinate(DIG)and the potential mechanism on alcoholic fatty liver cell model induced in vitro.Methods:MTT assay was used to screen the optimal concentration of ethanol and DIG injection.Ultra-microstructure was observed under transmission electron microscope.Intracellular triglyceride concentration was measured by hol-automatic biochemistry.Expressions of peroxisome proliferator-activated receptor-gamma (PPARγ)

sterol regulatory element binding protein-1(SREBP-1)and SREBP cleavage activating protein(SCAP)were determined by immunocyto-chemistry(ICC).Results:1.The screened optimal DIG concentration was 2.273μg/ml.2.Masses of lipid droplets in cytoplasm were observed under transmission electron microscope.3.Hol-automatic biochemistry measured intracellular triglyceride concentration to be(1.84±0.16)mmol/L and(1.05±0.12)mmol/L respectively in Ld30 group and DIG group

and the difference was statistically significant(P<0.01).4.The expression of PPARγ

SREBP-1

and SCAP in DIG group decreased

as compared with Ld30 group

and the difference was statistically significant.Conclusion:DIG is helpful to relieve steatosis of alcoholic fatty liver induced in vitro

and its possible mechanism is by down-regulating expressions of PPARγ

SREBP-1

and SCAP

thus lipogensis is inhibited in the liver cells.

关键词

甘利欣甘油三酯酒精性脂肪肝

Keywords

Diammonium glycyrrhizinate injectionTriglyceride levelalcoholic fatty liver

references

Views

343

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of lycium barbarum polysaccharide on the expression of hepatocyte cytochrome P4502E1 in rats with alcoholic fatty liver

Effects of Lycium Barbarum Polysaccharide on the expression of hepatocyte cytochrome P4503A in rats with alcoholic fatty liver

Experimental study on hepatic-protective effects of Shuangge Jiecheng Pill in rats of alcoholic fatty liver

Effects of blueberry on the expression of TNF-α-R1 and SREBP-1c in acute alcoholic fatty liver

Related Author

GU Sai

et al

姜蓉

GU Sai

et al

LUO Huali

WANG Ying

Zhao Xueke

Related Institution

Department of Gastroenterology,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学组胚教研室

Department of Gastroenterology,the First Affilated Hospital,Chongqing Medical University

Department of Acupuncture and Massage College of Chinese Medicine，Chongqing Medical University

Department of Infectious Diseases，Affiliated Hospital of Guiyang Medical College

⁰