Effects of matrine in inducing Raji cell apoptosis and on the expression of Bcl-2

HUANG Xi; et al

doi:10.13406/j.cnki.cyxb.2010.02.002

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Effects of matrine in inducing Raji cell apoptosis and on the expression of Bcl-2

更新时间：2024-01-17

- Effects of matrine in inducing Raji cell apoptosis and on the expression of Bcl-2
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 189-191(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院血液内科
  2. 重庆医科大学基础医学院核医学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.002
  CLC： R285
- Published：2010，
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[1]黄曦,陈建斌,郭启帅,杨泽松,刘占术,黄宗干.苦参碱诱导Raji细胞凋亡及其对Bcl-2表达的影响[J].重庆医科大学学报,2010,35(02):189-191.

HUANG Xi, et al. Effects of matrine in inducing Raji cell apoptosis and on the expression of Bcl-2. [J]. Journal of Chongqing Medical University 35(2):189-191(2010)
[1]黄曦,陈建斌,郭启帅,杨泽松,刘占术,黄宗干.苦参碱诱导Raji细胞凋亡及其对Bcl-2表达的影响[J].重庆医科大学学报,2010,35(02):189-191. DOI： 10.13406/j.cnki.cyxb.2010.02.002.

HUANG Xi, et al. Effects of matrine in inducing Raji cell apoptosis and on the expression of Bcl-2. [J]. Journal of Chongqing Medical University 35(2):189-191(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.002.

摘要

目的:观察苦参碱（Matrine

Mat）对体外人淋巴瘤Raji细胞增殖及细胞凋亡的影响

探讨Mat诱导Raji细胞凋亡的作用机制。方法:四甲基偶氮唑蓝（MTT）比色法观察Mat对Raji细胞增殖的影响;流式细胞术检测凋亡率;免疫细胞化学技术检测Mat对Raji细胞株Bcl-2蛋白表达的影响;RT-PCR法检测Bcl-2 mRNA表达。结果:不同浓度的Mat对Raji细胞均有一定的增殖抑制作用

且呈量效和时效关系;Mat能明显诱导Raji细胞凋亡;免疫细胞化学结果显示Bcl-2在实验组中的表达显著低于对照组。RT-PCR显示Bcl-2 mRNA表达明显减弱

且随剂量增加越明显。结论:Mat能抑制人淋巴瘤Raji细胞的增殖

促进细胞凋亡

其作用机制可能与下调Bcl-2表达有关。

Abstract

Objective:To observe the effects of matrine on proliferation and apoptosis of human lymphoma cell line Raji in vitro

and to explore its mechanism.Methods:The proliferation inhibition rate of Raji cells was assayed by MTT analysis.Cell apoptosis rate was evaluated by flow cytometry.The expression level of Bcl-2 protein was evaluated by streptavidin-biotin-peroxidase method.The Bcl-2 mRNA expression of Raji cells treated with matrine was assayed by RT-PCR.Results:The matrine of different concentrations all had cytotoxic effects on Raji cells

in a dose-and time-dependent manner.The apoptosis induced by matrine was confirmed in Raji cells. Immunocytochemistry assessment showed the expression of Bcl-2 was significantly down-regulated after treated with matrine.The Bcl-2 mRNA expression in Raji cells was decreased after treated by matrine

with a concentration-effect relationship.Conclusion:Matrine can inhibit the proliferation of Raji cells significantly.By down-regulating the expression of Bcl-2 and inducing the apoptosis.

关键词

苦参碱Raji细胞细胞增殖细胞凋亡

Keywords

MatrineRaji cellscell proliferationcell apoptosis

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