Establishment and drug-resistance gene expression of cisplatin-resistant cell line Acc-3/CDDP

ZHANG Fu-Jun; et al

doi:10.13406/j.cnki.cyxb.2010.01.048

您当前的位置：

首页 >

文章列表页 >

Establishment and drug-resistance gene expression of cisplatin-resistant cell line Acc-3/CDDP

更新时间：2024-01-17

- Establishment and drug-resistance gene expression of cisplatin-resistant cell line Acc-3/CDDP
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 54-56(2010)
- 作者机构：
  
  重庆医科大学附属第一医院口腔颌面外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.048
  CLC： R739.87
- Published：2010，
扫描看全文
[1]张福军,季平,杨凯,李雅冬.多药耐药口腔腺样囊性癌细胞株(Acc-3/CDDP)的建立及其细胞中相关耐药基因表达的变化[J].重庆医科大学学报,2010,35(01):54-56.

ZHANG Fu-Jun, et al. Establishment and drug-resistance gene expression of cisplatin-resistant cell line Acc-3/CDDP. [J]. Journal of Chongqing Medical University 35(1):54-56(2010)
[1]张福军,季平,杨凯,李雅冬.多药耐药口腔腺样囊性癌细胞株(Acc-3/CDDP)的建立及其细胞中相关耐药基因表达的变化[J].重庆医科大学学报,2010,35(01):54-56. DOI： 10.13406/j.cnki.cyxb.2010.01.048.

ZHANG Fu-Jun, et al. Establishment and drug-resistance gene expression of cisplatin-resistant cell line Acc-3/CDDP. [J]. Journal of Chongqing Medical University 35(1):54-56(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.048.

摘要

目的:采用细胞毒性化疗药物顺铂（Cisplatin

CDDP）诱导口腔腺样囊性癌Acc-3细胞系

得到耐药表型的细胞亚系Acc-3/CDDP

检测其多药耐药性、探讨与其亲代Acc-3细胞系的耐药相关基因变化。方法:用浓度递增法建立Acc-3/CDDP耐药细胞系;用MTT法检测Acc-3/CDDP细胞的耐药指数

RT-PCR检测多药耐药基因MDR-1、MRP-1、GST-π和Bcl-2基因的mRNA表达水平。结果:Acc-3/CDDP对CDDP、5-氟尿嘧啶（5-Fu）、长春新碱（VCR）、氨甲喋呤（MTX）和平阳霉素（PYM）的耐药指数（Resistancefactor

RF）分别为8.9、1.97、2.83、2.42、1.1;RT-PCR显示:Acc-3/CDDP中MRP-1、GST-π、Bcl-2较Acc-3升高

Acc-3/CDDP中有MDR-1基因表达

而Acc-3中无MDR-1基因表达。结论:MRP-1、GST-π、Bcl-2基因的表达水平在多药耐药口腔腺样囊性癌Acc-3/CDDP细胞中显著升高

这为进一步探讨肿瘤多药耐药的形成机制提供了科学基础。

Abstract

Objective:To establish a CDDP-resistant cell line from human oral adenoid cystic carcinoma cell line Acc-3 and compare the drug-resistance gene expression between them.Method:The CDDP-resistant cell line Acc-3/CDDP was established by increasing the concentration of CDDP added to Acc-3 cells step wisely.The index of drug-resistance was estimated by MTT assay.The expression of MDR-1

MRP-1

GST-πand Bcl-2 were measured byRT-PCR optical densityOD.Result:The resistance factor(sRF=IC5(0Acc-3/CDDP) /IC5(0Acc-3))for CDDP

5-Fu

VCR

MTX and PYMwere 8.9

1.97

2.83

2.42

1.1 respectively.The expression of drug-resistance gene MRP-1

GST-πand Bcl-2 increased in Acc-3/CDDP cells.MDR-1 was expressed in Acc-3/CDDP

but none in Acc-3.Conclusion:MDR-1

MRP-1

GST-πand Bcl-2 were significantly over expressed in CDDP-resistant cell line Acc-3/CDDP

which can provide effective models for the study on multi-drug resistance of oral carcinoma.

关键词

口腔腺样囊性癌细胞多药耐药基因

Keywords

OralcavityAdenoid cystic carcinoma cellMulti-drug resistancegene

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Bioinformatics analysis of differentially co-expressed genes in peripheral blood and hippocampal tissue of patients with Alzheimer’s disease

Effect of the interaction between Gly1057Asp in IRS2 gene and environment on the risk of type 2 diabetes

Expression of Bak and Bad gene in brain after global ischemia-reperfusion

Construction of a recombinant adenovirus vector containing HBX gene

Effect of titin gene on the procession of MSCs differentiating into cardiomyocytes in vitro

Related Author

Wu Xianbo

Chen Ziting

Chen Peiliang

Song Weiqi

Gao Jian

Wu Yinru

Zhong Yishi

Zhang Suhua

Related Institution

Department of Epidemiology，School of Public Health，Southern Medical University

Laboratory of Disorders Genes and Department of Pharmacology,Medicine School of Jishou University

Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education,Chongqing Medical University

Department of Endocrinology,The First Affiliated Hospital of Chongqing Medical University

四川省遂宁市人民医院

⁰