Up-regulation of tumor necrosis factor-α on c-jun in cultured human retinal pigment epithelial cells

WANG Jing-bo; et al

doi:10.13406/j.cnki.cyxb.2010.01.046

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Up-regulation of tumor necrosis factor-α on c-jun in cultured human retinal pigment epithelial cells

更新时间：2024-01-17

- Up-regulation of tumor necrosis factor-α on c-jun in cultured human retinal pigment epithelial cells
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 51-53(2010)
- 作者机构：
  
  1. 解放军第309医院眼科
  2. 第四军医大学西京医院眼科
  3. 山东省济南师范学校
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.046
  CLC： R774.1
- Published：2010，
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[1]王静波,惠延年,关娟,杨丽.肿瘤坏死因子上调人视网膜色素上皮细胞c-jun的表达[J].重庆医科大学学报,2010,35(01):51-53.

WANG Jing-bo, et al. Up-regulation of tumor necrosis factor-α on c-jun in cultured human retinal pigment epithelial cells. [J]. Journal of Chongqing Medical University 35(1):51-53(2010)
[1]王静波,惠延年,关娟,杨丽.肿瘤坏死因子上调人视网膜色素上皮细胞c-jun的表达[J].重庆医科大学学报,2010,35(01):51-53. DOI： 10.13406/j.cnki.cyxb.2010.01.046.

WANG Jing-bo, et al. Up-regulation of tumor necrosis factor-α on c-jun in cultured human retinal pigment epithelial cells. [J]. Journal of Chongqing Medical University 35(1):51-53(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.046.

摘要

目的:检测肿瘤坏死因子（Tumor necrosisfactor

TNF）-α对培养人视网膜色素上皮（Retinal pigment epithelium

RPE）细胞c-jun表达的影响。方法:40ng/ml TNF-α作用于体外培养人RPE细胞不同时间（0、10、20、40、60min）

采用免疫荧光染色法和逆转录聚合酶链反应（RT-PCR）观察c-jun蛋白和mRNA的表达变化。结果:在未受刺激的RPE细胞中

c-jun表达微弱或不表达

细胞浆和部分细胞核为浅黄绿色荧光。40ng/ml TNF-α刺激后

RPE细胞的荧光强度逐渐增强并出现核移位。随着TNF-α的作用

c-jun mRNA的表达开始逐渐增强

于40min时达峰值

随后表达略有减低。结论:TNF-α可在短期内迅速上调体外培养人RPE细胞c-jun蛋白和mRNA的表达

且蛋白表达出现核移位现象

提示TNF-α可引起RPE细胞c-jun的活化。

Abstract

Objective:To investigate the effect oftumor necrosis factor (TNF)-α on the expression ofc-jun in the cultured human retinal pigment epithelial(RPE)cells.Methods:RPE cells were exposed to 40 ng/ml TNF-α for 0

40 and 40 minutes

respectively.Immunofluorescence staining and reverse transcription polymerase chain reaction(RT-PCR)were used to detect the levels of c-jun protein and mRNA in RPE cells.Results:Untreated RPE cells revealed faint or none green fluorescence of c-jun in the cytoplasm and some nuclei.With exposure to TNF-α

c-jun increased obviously with stronger green fluorescence.With treatment of TNF-α

c-jun mRNA increased gradually and enhanced to the peak at 40 min.Conclusion:TNF-α can up-regulate c-jun protein and mRNA in cultured human RPE cells and induce its nuclear transposition

which suggests the activation of c-jun.

关键词

视网膜色素上皮肿瘤坏死因子c-Jun

Keywords

Retinal pigment epitheliumTumor necrosisfactorc-Jun

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