The study of amyotrophic lateral sclerosis like changes caused by chronic cadmium poisoning in rats

XIE Wu-tao; et al

doi:10.13406/j.cnki.cyxb.2010.01.027

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The study of amyotrophic lateral sclerosis like changes caused by chronic cadmium poisoning in rats

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- The study of amyotrophic lateral sclerosis like changes caused by chronic cadmium poisoning in rats
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 25-28(2010)
- 作者机构：
  
  重庆医科大学基础医学院电镜室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.027
  CLC： R595;R744.8
- Published：2010，
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[1]谢武桃,贺进,李庆春,邹聪,徐晨.慢性镉中毒致大鼠肌萎缩侧索硬化症样改变的研究[J].重庆医科大学学报,2010,35(01):25-28.

XIE Wu-tao, et al. The study of amyotrophic lateral sclerosis like changes caused by chronic cadmium poisoning in rats. [J]. Journal of Chongqing Medical University 35(1):25-28(2010)
[1]谢武桃,贺进,李庆春,邹聪,徐晨.慢性镉中毒致大鼠肌萎缩侧索硬化症样改变的研究[J].重庆医科大学学报,2010,35(01):25-28. DOI： 10.13406/j.cnki.cyxb.2010.01.027.

XIE Wu-tao, et al. The study of amyotrophic lateral sclerosis like changes caused by chronic cadmium poisoning in rats. [J]. Journal of Chongqing Medical University 35(1):25-28(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.027.

摘要

目的:探讨小剂量慢性镉中毒法建立大鼠肌萎缩侧索硬化症动物模型的可行性。方法:成年大鼠饮水慢性染镉

每天饮用含镉去离子水（150mg/L）

记录每天饮水量

120d后取材。采用神经电生理、光镜、电镜技术测定大鼠坐骨神经传导速度、股二头肌形态学变化、脊髓前角运动神经元形态学变化;同时采用原子吸收石墨炉法、生物化学技术测定脊髓内镉含量以及脊髓组织细胞内铜锌超氧化物歧化酶1（CuZn-SOD1）活性、丙二醛含量的变化。结果:染镉后120d

大鼠运动神经传导速度减慢但股二头肌纤维直径变化不明显;脊髓前角运动神经元胞体平均截面积有变小、神经元胞浆内粗面内质网排列紊乱并出现核糖体脱颗粒现象;染镉后脊髓内镉含量增加、脊髓组织匀浆内CuZn-SOD1酶活性降低

而丙二醛含量升高。结论:镉可以在成年大鼠脊髓内蓄积

导致前角运动神经元形态和功能损伤。提示慢性镉中毒法可用于建立大鼠肌萎缩侧索硬化症的动物模型。

Abstract

Objective:Tostudy the feasibility of making animal model ofamyotrophic lateral sclerosis usinglowdose chronic cadmium poisoning method in rats.Methods:Adult SD rats were treated with cadmium chloride(150mg/L)by drinking for 120 days.Motor nerve conduction velocity

the morphologieal changes of musculus biceps femoris and spinal motor neuron were studied with electrophysiolog-ical technique

light and electron microscopy.The content of cadmium in the spinal cord

the active change of CuZn-SOD1 and MDA were measured with the atomic absorption and biochemistry techniques .Results:120 days after the cadmium(Cd)treatment in rats

the motor nerve conduction velocity were slowdown in the Cd group.However

no obvious change was found in the muscle fiber diameter between Cd and control group.The mean cross-sectional areas of spinal motorneurons were decreased and the degranulation of ribosomes were found in some motorneurons.The content of Cd in spinal cord increased significantly after Cd exposure.The enzymatic activity of CuZn-SOD1 was weak and the content of MDA increased in Cd group.Conclusion:Chronic low dose Cd exposure can deposit in the spinal cord of adult rats.It may cause functional change in spinal motorneurons and motor nerve fibers

which can be the method to make the animal model of amyotrophic lateral sclerosis.

关键词

镉脊髓运动神经元肌萎缩侧索硬化症

Keywords

cadmiumSpinal cordMotorneuronsAmyotrophic lateral sclerosis

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