A correlative study between early vertebral canal decompression and apoptosis following spinal cord injury in the rats

LI Jing; et al

doi:10.13406/j.cnki.cyxb.2010.01.021

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A correlative study between early vertebral canal decompression and apoptosis following spinal cord injury in the rats

更新时间：2024-01-17

- A correlative study between early vertebral canal decompression and apoptosis following spinal cord injury in the rats
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 1-4(2010)
- 作者机构：
  
  重庆医科大学神经科学研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.021
  CLC： R651.3
- Published：2010，
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[1]李京,孙善全,朱淑娟,刘辉,张兴业,高宝兵.大鼠椎管减压与细胞凋亡的相关性研究[J].重庆医科大学学报,2010,35(01):1-4.

LI Jing, et al. A correlative study between early vertebral canal decompression and apoptosis following spinal cord injury in the rats. [J]. Journal of Chongqing Medical University 35(1):1-4(2010)
[1]李京,孙善全,朱淑娟,刘辉,张兴业,高宝兵.大鼠椎管减压与细胞凋亡的相关性研究[J].重庆医科大学学报,2010,35(01):1-4. DOI： 10.13406/j.cnki.cyxb.2010.01.021.

LI Jing, et al. A correlative study between early vertebral canal decompression and apoptosis following spinal cord injury in the rats. [J]. Journal of Chongqing Medical University 35(1):1-4(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.021.

摘要

目的:观察在大鼠脊髓压迫性损伤后椎管减压与神经细胞凋亡的关系。方法:健康成年SD大鼠36只

随机分为两组:（1）椎管减压组30只

每组10只

采用自制压迫装置于L1水平制做椎管减压模型;（2）假手术组6只

只做全椎板切除不做脊髓压迫。用HE、Nissl、TUNEL、免疫组化和Western blot等方法

分别于脊髓压迫6、12h和24h后去除压迫装置

观察减压段脊髓组织病理变化、细胞凋亡与caspase-3的表达变化情况。结果:TUNEL（+）细胞数于6h减压时开始增高（9.62±3.54）

24h减压时达峰值（26.09±4.11）

与假手术组（3.38±1.04）相比具有显著性差异（P<0.05）;各减压组间比较有显著性差异（P<0.05）。caspase-3免疫反应与TUNEL（+）细胞数量表达变化相符。结论:大鼠脊髓损伤后

神经细胞凋亡的改变与脊髓受压时间长度相关;早期进行椎管减压可减少继发性神经细胞凋亡

加快神经功能恢复。

Abstract

Objective:To investigate whether spinal cord decompression is related to neural cell apoptosis after spinal cord injury.Methods:The spinal cord was compressed posteriorly at L1 level using a self-designed compression device for 6

12 h and 24 h

followed by decompression by removal of the screw.Apoptosis and cellular damage were assessed by the staining of HE、Nissl、terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)and immunostaining of caspase-3.Results:Compared with shame-operation group

the number of TUNEL positive cells was significantly higher in the decompression at 6、12、24 h after spinal cord injury

which was consistent with immunoreactive to caspase-3(P<0.05).Conclusion:The results indicate that the dynamic change of apoptosis was correlated with duration of spinal compression.Early decompression could reduce neural cell apoptosis following secondary spinal cord injury.

关键词

脊髓损伤减压凋亡半胱氨酸蛋白酶3

Keywords

spinal cord injurydecompressionapoptosisCaspase-3

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