Experimental study of antiviral effects of recombinant eukaryotic plasmid PcDNA3.1-MxA on Hepatitis B virus

HUANG Qin; et al

doi:10.13406/j.cnki.cyxb.2010.01.016

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Experimental study of antiviral effects of recombinant eukaryotic plasmid PcDNA3.1-MxA on Hepatitis B virus

更新时间：2024-01-17

- Experimental study of antiviral effects of recombinant eukaryotic plasmid PcDNA3.1-MxA on Hepatitis B virus
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 46-48(2010)
- 作者机构：
  
  1. 重庆医科大学儿童医院传染科
  2. 重庆医科大学感染性疾病分子生物学教育部重点实验室
  3. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.016
  CLC： R512.62
- Published：2010，
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[1]黄琴,彭明利,赵瑞秋,刘爱平,任红,许红梅.人MxA基因重组真核载体抗乙型肝炎病毒作用的实验室研究[J].重庆医科大学学报,2010,35(01):46-48.

HUANG Qin, et al. Experimental study of antiviral effects of recombinant eukaryotic plasmid PcDNA3.1-MxA on Hepatitis B virus. [J]. Journal of Chongqing Medical University 35(1):46-48(2010)
[1]黄琴,彭明利,赵瑞秋,刘爱平,任红,许红梅.人MxA基因重组真核载体抗乙型肝炎病毒作用的实验室研究[J].重庆医科大学学报,2010,35(01):46-48. DOI： 10.13406/j.cnki.cyxb.2010.01.016.

HUANG Qin, et al. Experimental study of antiviral effects of recombinant eukaryotic plasmid PcDNA3.1-MxA on Hepatitis B virus. [J]. Journal of Chongqing Medical University 35(1):46-48(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.016.

摘要

目的:研究重组真核载体PcDNA3.1-MxA体外抗HBV的作用

为研究MxA基因（myxovirus-resistant A）治疗慢性HBV感染奠定基础。方法:将构建成功的重组真核载体PcDNA3.1-MxA转染入HepG2.2.15。将HepG2.2.15分为实验组（转染PcDNA3.1-MxA的HepG2.2.15）和对照组（转染PcDNA3.1的HepG2.2.15）

应用RT-PCR方法检测两组HepG2.2.15内MxA mRNA（P<0.01）

并应用ELISA法检测两组HepG2.2.15内HBsAg、HBeAg的水平。结果:RT-PCR扩增结果显示实验组的MxA mRNA水平均较对照组明显升高

实验组HBsAg、HBeAg水平明显低于对照组（均P<0.01）。结论:转染重组真核载体PcDNA3.1-Mx AHepG2.2.15内MxA蛋白明显抑制了HBV DNA的复制及表达

为进一步研究MxA蛋白体内抗病毒作用提供理论。

Abstract

Objective:To investigate the anti-HBVeffects of the recombinant eukaryotic plasmid PcDNA3.1-MxAin HepG2.2.15 cells in vitro.Methods:The recombinant eukaryotic plasmid PcDNA3.1-MxA was transfected into HepG 2.2.15 cells with Lipofection after extracted and confirmed with restrictive endonuclease analysis.The control group was HepG2.2.15 cells transfected with PcDNA3.1.The levels of MxA mRNA were detected by RT-PCR and the titers of HBsAg and HBeAg in supernatant liquids were detected by ELISA.Results:The RT-PCR results showed the level of MxA mRNA in the treatment group was much higher than that in the control group.The titers ofHBsAgand HBeAgin supernatant liquid ofthe treatment group were significantlylower than those in the control group cells(P<0.01).Conclusion:The study indicates that MxA protein can inhibit replication and expression of HBV.

关键词

PcDNA3.1-MxAHepG2.2.15HBV复制及表达

Keywords

PcDNA3.1-MxAHepG2.2.15Replication and expression of HBV

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Construction and identification of the recombinant eucaryotic plasmid PcDNA3.1-MxA

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HUANG Qin

et al

刘爱平

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Related Institution

Department of Infectious Diseases and Gastroenterology,the Children’s Hospital, Chongqing Medical University

重庆医科大学附属儿童医院感染消化科

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