Effect of chemokines,activity enhanced by mobilization of bone marrow cells on solution and absorption of venous thrombosis

SUN Hao

doi:10.13406/j.cnki.cyxb.2010.01.009

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Effect of chemokines,activity enhanced by mobilization of bone marrow cells on solution and absorption of venous thrombosis

更新时间：2024-01-17

- Effect of chemokines,activity enhanced by mobilization of bone marrow cells on solution and absorption of venous thrombosis
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 110-113(2010)
- 作者机构：
  
  重庆市肿瘤研究所普通外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.009
  CLC： R543
- Published：2010，
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[1]孙浩.骨髓细胞动员增强趋化因子活性对静脉血栓溶解吸收的影响[J].重庆医科大学学报,2010,35(01):110-113.

SUN Hao. Effect of chemokines,activity enhanced by mobilization of bone marrow cells on solution and absorption of venous thrombosis. [J]. Journal of Chongqing Medical University 35(1):110-113(2010)
[1]孙浩.骨髓细胞动员增强趋化因子活性对静脉血栓溶解吸收的影响[J].重庆医科大学学报,2010,35(01):110-113. DOI： 10.13406/j.cnki.cyxb.2010.01.009.

SUN Hao. Effect of chemokines,activity enhanced by mobilization of bone marrow cells on solution and absorption of venous thrombosis. [J]. Journal of Chongqing Medical University 35(1):110-113(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.009.

摘要

目的:探讨骨髓细胞动员对巨噬细胞聚集静脉血栓趋化作用的影响。方法:SD成年大鼠随机分为正常组、假手术组、血栓组和rhG-CSF组。术前0.5h、术后7d和14d采集外周血

用ELISA法检测外周血MCP-3水平。术后7d、14d取血栓组和rhG-CSF组下腔静脉标本

用RT-PCR法检测血栓中MCP-3mRNA的表达情况。结果:术后14drhG-CSF组外周血MCP-3水平明显高于术前

且高于假手术组和血栓组术后14d外周血MCP-3水平

分别与术前、假手术组和血栓组比较P<0.05

差异有统计学意义。术后7d和14d

rhG-CSF组MCP-3mRNA表达较血栓组显著升高

两组间比较有显著差异P<0.01。结论:骨髓细胞动员增强了MCP-3对巨噬细胞的趋化作用。

Abstract

Objective:To discuss the effect of bone marrow mobilization on chemotaxis of aggregation of macrophages in venous thrombosis.Methods:SD adult rats were divided randomly into normal group

sham operation group

thrombus group and rhG-CSF group.Peripheral blood was harvested at 30min before operation and on the 7th and 14th day after operation.Level of MCP-3 in peripheral blood was detected by ELISA.Inferior vena cava specimens of thromus group and rhG-CSF group were collected at postoperative 7th and 14th day.With the application of RT-PCR

expression of MCP-3mRNA in thrombus was detected.Results:The level of MCP-3 in peripheral blood of rhG-CSF group was significantly higher than that before operation and also higher than the levels of MCP-3 in peripheral blood of sham operation group and thrombus group on the 14th day after operation(P<0.05)

and the difference was significant.On the 7th and 14th day after operation

compared with thrombus group

the expression of MCP-3 mRNA in rhG-CSF group remarkablely increased

and the difference was significant beween these two groups(P<0.01).Conclusion:Brone marrow cell mobilization enhances the chemotaxis of MCP-3 on macrophages.

关键词

骨髓细胞动员单核细胞趋化因子-3巨噬细胞

Keywords

BonemarrowcellmobilizationMCP-3macrophage

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