Comparison of two methods to purfy rat follicle stem cell

LU Wei; et al

doi:10.13406/j.cnki.cyxb.2010.01.002

您当前的位置：

首页 >

文章列表页 >

Comparison of two methods to purfy rat follicle stem cell

更新时间：2024-01-17

- Comparison of two methods to purfy rat follicle stem cell
- Journal of Chongqing Medical University Vol. 35, Issue 1, Pages: 124-126(2010)
- 作者机构：
  
  重庆医科大学附属第一医院皮肤性病科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.01.002
  CLC： R329
- Published：2010，
扫描看全文
[1]卢葳,陈瑾,李惠.两种纯化大鼠毛囊干细胞方法的比较[J].重庆医科大学学报,2010,35(01):124-126.

LU Wei, et al. Comparison of two methods to purfy rat follicle stem cell. [J]. Journal of Chongqing Medical University 35(1):124-126(2010)
[1]卢葳,陈瑾,李惠.两种纯化大鼠毛囊干细胞方法的比较[J].重庆医科大学学报,2010,35(01):124-126. DOI： 10.13406/j.cnki.cyxb.2010.01.002.

LU Wei, et al. Comparison of two methods to purfy rat follicle stem cell. [J]. Journal of Chongqing Medical University 35(1):124-126(2010) DOI： 10.13406/j.cnki.cyxb.2010.01.002.

摘要

目的:显微分离技术分别与流式细胞仪分选和免疫磁珠分选两种方法联合使用

比较不同方法的优缺点

以进一步探索毛囊干细胞的体外分选培养的条件。方法:显微分离鼠毛囊隆突部

消化成单细胞悬液。分别利用流式细胞仪和免疫磁珠分选毛囊干细胞。观察细胞形态并检测分选前后细胞的纯度

活性

回收率

进行免疫荧光鉴定。结果:两种方法均能分选出实验所需的毛囊干细胞。流式细胞仪分选细胞纯度高

活性稍弱;免疫磁珠法分选细胞纯度稍低但活性强。结论:流式细胞仪分选和免疫磁珠分选均能有效获取毛囊干细胞。显微分离技术可根据实验需求选择不同的分选方法搭配。

Abstract

Objective:To compare the advantage and disadvantage of two methed purfy rat follicle stem cells by microdissection and Vario magnetic activated cell sorting(MACS) or flowcytometry in combination.in order to further explore the rat follicle stem cells in vitro culture conditions.Methods:rat follicle bulg digested into cell suspension after obtained by microdissection.Observation of cell morphology and to detect cells before and after sorting the purity

activity

recovery

identification by immunofluorescence.Results:All Two methods can be purfy rat follicle stem cell.By flow cytometry sorting cells with high purity and low activity than MACS sorting cell.Conclusion:flow cytometry and MACS both can effectively obtain the hair follicle stem cells.Microdissection combine which method depend on the experimental requirements.

关键词

毛囊干细胞纯化免疫磁珠流式细胞术

Keywords

Follicle stem cellpurificationImmunomagnetic beadsFlowcytometry

references

Views

258

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Methodology of separation, purification, cultivation and identification of rat bone marrow macrophage

Over-expression and purification of recombinant spike protein fragment of SARS coronavirus

High lever soluble expression and purification of recombinant human IL-18 in E.coli

The immunoactivity study of purified recombinant CP₄-EPSPS protein

Expression,identification and purification of the resuscitation-promoting factor B of Mycobacterium tuberculosis

Related Author

et al

LI Jing

黄爱龙

郑建

王丕龙

唐霓

张秉强

闫歌

Related Institution

Department of Histology and Embryology, College of Basic Medical Sciences, Chongqing Medical University

重庆医科大学基础医学院组胚教研室

Institute of Viral Hepatitis,Chongqing Medical University

重庆医科大学病毒性肝炎研究所

Key Laboratory of Biochemistry and Molecular Pharmacolog,Chongqing Medical University

⁰