Determination of serumα-fetoprotein by TRFIA,RIA and ELISA

CHEN Hai-wei

doi:10.13406/j.cnki.cyxb.2008.05.030

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Determination of serumα-fetoprotein by TRFIA,RIA and ELISA

更新时间：2024-01-17

- Determination of serumα-fetoprotein by TRFIA,RIA and ELISA
- Journal of Chongqing Medical University Issue 5, Pages: 629-630(2008)
- 作者机构：
  
  1. 中南大学湘雅三医院检验科
  2. 中南大学湘雅三医院检验科,长沙,410003
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.030
  CLC： R446.6;R817.4
- Published：2008，
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[1]陈海蔚,伍勇.3种不同方法检测甲胎蛋白的比较[J].重庆医科大学学报,2008(05):629-630+640.

CHEN Hai-wei. Determination of serumα-fetoprotein by TRFIA,RIA and ELISA. [J]. Journal of Chongqing Medical University (5):629-630(2008)
[1]陈海蔚,伍勇.3种不同方法检测甲胎蛋白的比较[J].重庆医科大学学报,2008(05):629-630+640. DOI： 10.13406/j.cnki.cyxb.2008.05.030.

CHEN Hai-wei. Determination of serumα-fetoprotein by TRFIA,RIA and ELISA. [J]. Journal of Chongqing Medical University (5):629-630(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.030.

摘要

目的:比较测定甲胎蛋白（Alpha-fetoprotein

AFP）的3种不同方法。方法:采用时间分辨荧光免疫分析法（Time-re- solved fluoroimmuno assay

TRFIA）、放射免疫法（Radio immuno assay

RIA）与酶联免疫吸附法（Enzme linked immunoabsorbent assay

ELISA）。分别进行线性、精密度、对比实验比较。结果:3种方法中

TRFIA法线性范围最宽;精密度试验中TRFIA法批内为3.3%

批间为4.7%;RIA法批内为5.3%

批间为8.9%;ELISA法批内为10.5%

批间为15.2%。比较试验显示RIA法与TRFIA法的结果相关性比ELISA法与RIA法好。结论:TRFIA法测定AFP优于RIA法和ELISA法。

Abstract

Objective:To assess the efficacy of time-resolved fluoroimmuno assay(TRFIA) for quantative determination ofα-fetoprotein(AFP) in comparison with radio immuno assay(RIA) and enzme linked immunoabsorbent assay(ELISA). Methods:Thirty serum samples from randomly selected patients were collected to test AFP content with TRFIA

RIA and ELISA respectively.Results:The linear range of AFP was 1 to l 000 ng/ml for TRFIA

5～400ng/ml for RIA and 5～300ng/ml for ELISA.The intraassay coefficients of variation(CV) and interassay CV were 3.3% and 4.7% for TRFIA

5.3% and 8.9% for RIA

and 10.5% and 15.2% for ELISA

respectively.Correlation tests revealed that the results of RIA were more closely correlated with those of TRFIA than with those of ELISA.Conclusion:TRFIA is shown to be a better method for AFP quantitation than RIA and ELISA in terms of precision and linear range.

关键词

甲胎蛋白时间分辨荧光免疫分析法放射免疫法酶联免疫吸附法

Keywords

Alpha-fetoproteinsTime-resolved fluoroimmuno assayRadio-immuno assayEnzme-linked immunoabsorbent assay

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