Effbct of silencing PAR1 by RNA interference on invasive potential of human colon cancer cell lines

LU Xin-yang

doi:10.13406/j.cnki.cyxb.2008.05.024

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Effbct of silencing PAR1 by RNA interference on invasive potential of human colon cancer cell lines

更新时间：2024-01-17

- Effbct of silencing PAR1 by RNA interference on invasive potential of human colon cancer cell lines
- Journal of Chongqing Medical University Issue 5, Pages: 608-611(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院普外科
  2. 重庆医科大学附属第二医院普外科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.024
  CLC： R735.34
- Published：2008，
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[1]陆信仰,王继见,王亚旭.siRNA沉默PAR1基因表达对大肠癌细胞侵袭力的影响[J].重庆医科大学学报,2008(05):608-611.

LU Xin-yang. Effbct of silencing PAR1 by RNA interference on invasive potential of human colon cancer cell lines. [J]. Journal of Chongqing Medical University (5):608-611(2008)
[1]陆信仰,王继见,王亚旭.siRNA沉默PAR1基因表达对大肠癌细胞侵袭力的影响[J].重庆医科大学学报,2008(05):608-611. DOI： 10.13406/j.cnki.cyxb.2008.05.024.

LU Xin-yang. Effbct of silencing PAR1 by RNA interference on invasive potential of human colon cancer cell lines. [J]. Journal of Chongqing Medical University (5):608-611(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.024.

摘要

目的:研究RNA干扰活性蛋白酶受体（Protease-activated receptors

PAR1）表达以及对人类大肠癌细胞侵袭力的影响。方法:体外化学合成PAR1基因序列特异性双链RNA（dsRNA）

在脂质体（Lipid）的介导下转染入人大肠癌Lovo细胞株

荧光倒置显微镜下观察转染效率

应用RT-PCR和Western blot检测PAR1mRNA和蛋白表达

体外实验测定肿瘤细胞穿透matrigel的能力。结果:干扰片段转染组的转染Pgenesil-1-PAR1-siRNA后大肠癌Lovo细胞株的PAR1基因和蛋白的表达被PAR1-siRNA有效沉默

与阴性对照组、空白质粒转染组相比PAR1 mRNA和蛋白表达水平明显降低（P<0.05）。转染48h后测定大肠癌Lovo细胞的侵袭力明显降低（P<0.01）。结论:PAR1-siRNA能有效抑制人大肠癌细胞株PAR1基因和蛋白的表达

降低人大肠癌细胞的侵袭力。

Abstract

Objective:To study the effect of RNA interference(RNAi) on PAR1 gene mRNA expression and the invasive petential of human colon cancer cell lines.Methods:Chemically synthesizeddouble stranded RNA(dsRNA) targeting PAR1 was transfect- ed into human colon cancer cell Lovo using SiPORT Lipid.The transfection eficiency was observed under fluorescence confocal microscopy.Expression of PAR1 mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.Cell penetrate matrigel capacity were determined by invitro experiment.Results:PAR1-siRNA effectively in- hibited PAR1 mRNA and protion expression(P<0.05)

and degraded invasion capability of Lovo cells(P<0.01).Conclusion: PAR1-siRNA inhibits PAR1 mRNA and protein expression

degrades invasion of human colon cancer cell.

关键词

大肠癌RNA干扰活性蛋白酶受体侵袭

Keywords

Colon cancerRNAiProtease-activated receptorsInvasive

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