Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC)

ZHANG Cheng

doi:10.13406/j.cnki.cyxb.2008.05.022

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Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC)

更新时间：2024-01-17

- Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC)
- Journal of Chongqing Medical University Issue 5, Pages: 578-580(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院胸心外科
  2. 军事医学科学院放射与辐射医学研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.022
  CLC： R734.2
- Published：2008，
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[1]张诚,吴庆琛,李强,朱茂祥,杨陟华,潘秀颉,谢俊豪.烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中p16基因甲基化改变的研究[J].重庆医科大学学报,2008(05):578-580+591.

ZHANG Cheng. Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC). [J]. Journal of Chongqing Medical University (5):578-580(2008)
[1]张诚,吴庆琛,李强,朱茂祥,杨陟华,潘秀颉,谢俊豪.烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中p16基因甲基化改变的研究[J].重庆医科大学学报,2008(05):578-580+591. DOI： 10.13406/j.cnki.cyxb.2008.05.022.

ZHANG Cheng. Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC). [J]. Journal of Chongqing Medical University (5):578-580(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.022.

摘要

目的:探讨永生化人支气管上皮细胞（HPV-18 immortalized human bronchial epithelial cells

BEP-2D）恶性转化过程中p16基因甲基化改变。方法:选取正常BEP-2D细胞及经过烟草悬凝物处理后的15周（week-15

W-15）、25周（W-25）、38周（W-38）和43周（W-43）的BEP-2D细胞作为研究对象

采用巢式MSP（Nested-methylation-specific PCR

Nested-MSP）检测各细胞株中p16基因的甲基化改变情况。结果:正常BEP-2D细胞p16基因未发生甲基化

而烟草悬凝物处理后的15周、25周、38周和43周的BEP-2D细胞p16基因发生甲基化。结论:p16基因甲基化改变可能发生在肺癌形成的早期

检测p16基因甲基化可以作为肺癌早期辅助诊断的生物标记物之一。

Abstract

Objective:To study p16 methylation of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cells and BEP-2D cells treated by cigarette smoke condensate(CSC) for 15 weeks(W-15)

25 weeks(W-25)

38 weeks(W-38) and 43 weeks(W-43)respectively were chosen to study the p16 methylation status by Nested methylation specific PCR (Nested-MSP).Results:The p16 methylation was found in W-15

W-25

W-38 and W-43 BEP-2D cells

but not in the normal BEP-2D cell.Conclusion:The p16 methylation may be considered as a kind of biology-marker of the early diagnosis of lung cancer because the p16 methylation occurs in the earlier period of lung cancer.

关键词

烟草悬凝物P16甲基化巢式PCR

Keywords

Cigarette Smoke CondensateP16methylationNested-MSP

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