Establishment and evaluation of VX2 lung cancer model(squamous cancer) in rabbits

ZHANG Hui-jun

doi:10.13406/j.cnki.cyxb.2008.05.019

您当前的位置：

首页 >

文章列表页 >

Establishment and evaluation of VX2 lung cancer model(squamous cancer) in rabbits

更新时间：2024-01-17

- Establishment and evaluation of VX2 lung cancer model(squamous cancer) in rabbits
- Journal of Chongqing Medical University Issue 5, Pages: 541-545(2008)
- 作者机构：
  
  1. 重庆医科大学基础医学院生理教研室
  2. 重庆医科大学基础医学院生理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.019
  CLC： R734.2
- Published：2008，
扫描看全文
[1]张会军,王莎莉.兔VX2肺癌(鳞癌)模型的建立及鉴定[J].重庆医科大学学报,2008(05):541-545.

ZHANG Hui-jun. Establishment and evaluation of VX2 lung cancer model(squamous cancer) in rabbits. [J]. Journal of Chongqing Medical University (5):541-545(2008)
[1]张会军,王莎莉.兔VX2肺癌(鳞癌)模型的建立及鉴定[J].重庆医科大学学报,2008(05):541-545. DOI： 10.13406/j.cnki.cyxb.2008.05.019.

ZHANG Hui-jun. Establishment and evaluation of VX2 lung cancer model(squamous cancer) in rabbits. [J]. Journal of Chongqing Medical University (5):541-545(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.019.

摘要

目的:探讨兔VX2肺鳞癌模型的建立方法及其生物学特性。方法:自身接种传代保存的VX2肿瘤组织块

移植到健康兔肺内

建立兔肺鳞癌模型。通过肺部X片观察肿瘤生长情况

待其不同时间死亡后进行大体标本肺、心、肝、肾、脑和淋巴结形态学观察。结果:（1）建模成功率为100%。（2）形态学显示肺部肿瘤呈弥漫性分布

大小不等

呈结节样

表面鱼肉样。接种肿瘤6d时除脑组织外

心脏、淋巴结、肝脏和肾脏均见明显的转移瘤

并呈侵润性生长。（3）病理组织学和免疫组化证实为来源于上皮组织的鳞状细胞癌。结论:采用组织块移植法建造兔VX2肿瘤动物模型

不仅具有方法简单、无感染和成瘤率高的特点

而且VX2肿瘤的生物学行为与人类肿瘤相似

恶性程度高

对于大型动物建立肺癌模型具有较高的指导意义。

Abstract

Objective:To evaluate the establishment method and bionomics of VX2 lung cancer models in rabbits.Methods: VX2 tumor fragments

preserved from autoinculation rabbits into the lungs of healthy rabbits to establish the models

and then the tumorous growth was observed through the imageology and the morphology of the samples including the lung

the cardia

the liver

the kidney

the brain and lymphatic gland after death at different time.Results:(1) the achievement ratio of modeling was 100%。(2)The VX2 tumor discovered evidently in lung were displayed for diffuse

inequality of size

tuberculum and fish surface.It was visible that metastatic tumor

which were invasion

were discovered evidently in all six organs except for the brain after vaccination for six days.(3)Squamous cell carcinoma

which was originated from epithelial tissue

was confirmed through pathologic histology and immunohistochemistey.Conclusions:The construction of VX2 tumor animal models in rabbits by applying organism fragments not only is simple in method

free from infection and high in achievement ratio but also the biological behaviour of VX2 is similar to human tumors

with high level of malignancy.This study provides valuable guidance for the construction of the large animal models of the lung cancer.

关键词

VX2肺癌模型

Keywords

VX2lung cancermodel

references

Views

163

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Glycyrrhiza uralensis Fisch. endophytes combined with cisplatin on proliferation and apoptosis of A549 cells

Establishment and validation prediction models of benign and malignant predictors for solitary pulmonary nodules

Clinical analysis of dermatomyositis/polymyositis complicated with lung disease

Effect of progressive muscle relaxation training on coping style and emotion of patients with lung cancer

Related Author

Zhang Shanglong

Zhang Nan

Lian Xiaolong

Ye Liqiao

Ma Quhuan

Deng Yi

Zhu Xiaoqian

Zheng Li

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Department of Respiratory and Critical Care Medicine, Chongqing Medical University

State Key Lab. of Hydraulics and Mountain River Eng., Sichuan Univ.

Guodian Dadu River Basin Hydropower Development Co. Ltd.

Guodian Dadu River Dagangshan Power Generation Co. Ltd., Ya’an

⁰