Observation of circulating tumor cells in patients with lung carcinoma by real-time fluorescent quantitative-PCR approach before and after chemotherapy

Wang Mei

doi:10.13406/j.cnki.cyxb.2008.05.017

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Observation of circulating tumor cells in patients with lung carcinoma by real-time fluorescent quantitative-PCR approach before and after chemotherapy

更新时间：2024-01-17

- Observation of circulating tumor cells in patients with lung carcinoma by real-time fluorescent quantitative-PCR approach before and after chemotherapy
- Journal of Chongqing Medical University Issue 5, Pages: 526-530(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院检验科
  2. 重庆医科大学附属第一医院胸外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.017
  CLC： R734.2
- Published：2008，
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[1]王梅,葛明建.化疗前后肺癌患者外周循环癌细胞数的动态观察[J].重庆医科大学学报,2008(05):526-530+535.

Wang Mei. Observation of circulating tumor cells in patients with lung carcinoma by real-time fluorescent quantitative-PCR approach before and after chemotherapy. [J]. Journal of Chongqing Medical University (5):526-530(2008)
[1]王梅,葛明建.化疗前后肺癌患者外周循环癌细胞数的动态观察[J].重庆医科大学学报,2008(05):526-530+535. DOI： 10.13406/j.cnki.cyxb.2008.05.017.

Wang Mei. Observation of circulating tumor cells in patients with lung carcinoma by real-time fluorescent quantitative-PCR approach before and after chemotherapy. [J]. Journal of Chongqing Medical University (5):526-530(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.017.

摘要

目的:本实验观察32例肺癌患者化疗前后外周循环癌细胞数（CTCs）的动态变化

以寻找新化疗的疗效评判标准。方法:分别于化疗前及化疗1周期后、第3周收集32例肺癌患者外周血标本。同时行胸片或胸部CT扫描

并计算肿瘤的大小。运用实时荧光定量逆录-聚合酶链反应（fqRT-PCR）法定量检测外周血中CK19基因的表达。结果:化疗后CTCs水平显著降低（P=0.000 1）。化疗前小细胞肺癌（Small cell lung cancer

SCLC）患者外周血CK19 mRNA水平明显高于非小细胞肺癌（Non-small cell lung cancer

NSCLC）患者（P=0.041）。治疗前后CK19 mRNA测值变化与瘤负荷间存在正相关（P=0.000）。结论:在治疗过程中

运用fqRT-PCR法对肺癌患者CTCs进行定量监测是可行的。通过动态检测可及时评价化疗反应

有助于在早期阶段即对患者设计更合理、更有针对性的治疗方案。

Abstract

Objective:In order to develop a new standard of curative effect

this study was designed to track the number of CTCs in patients with lung cancer during chemotherapy.Methods:Samples of peripheral blood was taken from each lung cancer patients(n=32) on the day before chemotherapy as well as the third week after the chemotherapy cycle.The samples were subjected to real-time fqRT-PCR.Meanwhile the tumour size was determined by chest X-ray or computed tomograghy. Results:Compared to that of pre-chemotherapy

the expression level of CK19 in the patients significantly declined after chemotherapy(t=4.659

P=0.000 1).The level of CK19 mRNA in patients with SCLC was higher than that of patients with NSCLC(t=1.944

P=0.041).The change of CK19 mRNA correlated well with the type during the treatment.Relatively the shift of SCLC is more obvious(t=6.073

P=0.000 1).Conclusions:The real-time fluorescent quantitative-PCR approach is useful for measuring the relative number of CTCs in a patients’ peripheral blood to monitor the effectiveness of treatment

and for designing more comprehensive and reasonable therapeutic regimes at earlier dates for patients.

关键词

肺肿瘤聚合酶链反应细胞角蛋白信使RNA化学治疗

Keywords

lung neoplasmPolymerase chain reactionCytokeratinMessenger RNAchemotherapy

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