The tumor necrosis factor-αinhibits retinol binding protein 4 secretion in mature 3T3-L1 adipocytes

WEI You-neng

doi:10.13406/j.cnki.cyxb.2008.05.011

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The tumor necrosis factor-αinhibits retinol binding protein 4 secretion in mature 3T3-L1 adipocytes

更新时间：2024-01-17

- The tumor necrosis factor-αinhibits retinol binding protein 4 secretion in mature 3T3-L1 adipocytes
- Journal of Chongqing Medical University Issue 5, Pages: 575-577(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院内分泌科
  2. 重庆医科大学附属第一医院内分泌科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.011
  CLC： R589.2
- Published：2008，
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[1]未友能,青华,段炼,梅玫,程庆丰,李启富.TNF-α抑制成熟3T3-L1脂肪细胞分泌RBP4[J].重庆医科大学学报,2008(05):575-577+591.

WEI You-neng. The tumor necrosis factor-αinhibits retinol binding protein 4 secretion in mature 3T3-L1 adipocytes. [J]. Journal of Chongqing Medical University (5):575-577(2008)
[1]未友能,青华,段炼,梅玫,程庆丰,李启富.TNF-α抑制成熟3T3-L1脂肪细胞分泌RBP4[J].重庆医科大学学报,2008(05):575-577+591. DOI： 10.13406/j.cnki.cyxb.2008.05.011.

WEI You-neng. The tumor necrosis factor-αinhibits retinol binding protein 4 secretion in mature 3T3-L1 adipocytes. [J]. Journal of Chongqing Medical University (5):575-577(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.011.

摘要

目的:观察肿瘤坏死因子-α（TNF-α）对体外培养的成熟3T3-L1脂肪细胞分泌视黄醛结合蛋白4（Retinol Binding Protein 4

RBP4）的影响。方法:体外培养3T3-L1细胞

待细胞完全融合后两天进行诱导分化为成熟脂肪细胞

应用不同浓度的重组TNF-α干预

采用放射免疫分析法检测干预后不同时间成熟脂肪细胞RBP4的分泌量。结果:（1）不同浓度TNF-α（0.2、1.0、5.0、10ng/ml）对脂肪细胞RBP4的分泌均有抑制作用

其抑制效应呈剂量依赖性:随着TNF-α浓度的增加

RBP4水平降低越加明显。（2）各实验组随着干预时间延长

RBP4虽有降低之趋势

但组内各时间点差异无统计学差异。（3）二元回归分析提示TNF-α对RBP4有影响。结论:TNF-α能抑制成熟脂肪细胞RBP4的分泌

其抑制效应有剂量依赖性

而与干预时间无关。

Abstract

Objective:To explore the effects of tumor necrosis factor-α(TNF-α) on retinol binding protein 4 (RBP4) secreted by mature 3T3-L1 adipocytes in-vitro.Methods:3T3-L1 preadipocytes were cultured and differentiated into the mature adipocytes after 2 days of confluenence.Recombinant TNF-αat different concentrations was added to the culture medium of fully differentiated adipocytes.Total protein of RBP4 secreted by these adipocytes was evaluated by radioimmunoassay(RIA) at various times.Results:(1)Treatment of mature 3T3-L1 adipocytes with TNF-αat different concentrations (0.2

1.0

5.0

10ng/ml) decreased RBP4 secretion.The inhibitive effect of TNF-αon RBP4 was in dose dependent manner.The level of RBP4 decreased along with the elevated TNF-αconcentration in the culture medium.(2)Almost the RBP4 secretion in all experiment groups had a decreasing tread at different time points

but there was no statistical difference in each group.(3) Regression analysis indicated TNF-αhad significant associations with RBP4.Conclusion:TNF-αinhibits mature adipocytes RBP4 secretion in-vitro in dose-response effects

but there is no time-response effects.

关键词

视黄醛结合蛋白4肿瘤坏死因子-α脂肪细胞

Keywords

Retinol binding protein 4(RBP4)tumor necrosis factor-α(TNF-α)adipocyte

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