The study on feasibility of determinating the polysaccharide isolated from bletilla striata using dextron T20 as standard substance

KUANG Fu

doi:10.13406/j.cnki.cyxb.2008.05.003

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The study on feasibility of determinating the polysaccharide isolated from bletilla striata using dextron T20 as standard substance

更新时间：2024-01-17

- The study on feasibility of determinating the polysaccharide isolated from bletilla striata using dextron T20 as standard substance
- Journal of Chongqing Medical University Issue 5, Pages: 570-574(2008)
- 作者机构：
  
  1. 重庆医科大学药学院药剂教研室
  2. 重庆医科大学药学院药剂教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.05.003
  CLC： R284
- Published：2008，
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[1]匡扶,朱照静,马俐丽,程安媛.葡聚糖T20作为白芨多糖含量测定标准品可行性研究[J].重庆医科大学学报,2008(05):570-574.

KUANG Fu. The study on feasibility of determinating the polysaccharide isolated from bletilla striata using dextron T20 as standard substance. [J]. Journal of Chongqing Medical University (5):570-574(2008)
[1]匡扶,朱照静,马俐丽,程安媛.葡聚糖T20作为白芨多糖含量测定标准品可行性研究[J].重庆医科大学学报,2008(05):570-574. DOI： 10.13406/j.cnki.cyxb.2008.05.003.

KUANG Fu. The study on feasibility of determinating the polysaccharide isolated from bletilla striata using dextron T20 as standard substance. [J]. Journal of Chongqing Medical University (5):570-574(2008) DOI： 10.13406/j.cnki.cyxb.2008.05.003.

摘要

目的:采用星点设计-效应面法（Central composite design-response surface methodology

CCD-RSM）

考察蒽酮-硫酸法测量白芨多糖含量的可行性和适用条件。方法:以葡聚糖T20为标准品

以多糖溶液与蒽酮试剂比例、水解显色时间、室温冷却时间为自变量

621.0nm处的吸光度为因变量

对自变量各水平进行多元线性回归和二项式拟合

考察因变量和自变量间的相互关系

采用效应面法优化蒽酮-硫酸法测量白芨多糖含量的适用条件。结果:当多糖溶液与蒽酮试剂比例为1:2

水解显色时间8.00min

室温冷却放置15.00min时

测定葡聚糖T20和白芨多糖含量具有良好的相关性。结论:星点设计-效应面法可以用于评估以葡聚糖T20为标准品、蒽酮-硫酸法测量白芨多糖含量的可行性

并优化测量含量的适用条件。

Abstract

Objective:To optimize the condition of anthrone-sulfuric acid method to determine the polysaccharide isolated from Bletilla striata by central composite design/response surface methodology.Methods:Dextron T20 was a standard substance

independent variables were the ratio of the polysaccharide and anthrone-sulfuric acid

the time of color hydrolysis and the cooling time at room temperature

dependent variable was the UV absorption

nonlinear mathematic models were used to estimate the relationship between independent and dependent variables.Response surface methodology was used to optimize be condition of determination.Results:The optimum conditions of determination:the volume ratio of the polysaccharide and anthrone-sulfuric acid is 1:2;the time of color hydrolysis is 8.00 min;the cooling time at room temperature is 15.00min.On this condition

there was favorable correlation between the absorption of Dextron T20 and polysaccharide of Bletilla striata.Conclusions: Central composite design/response surface methodology can be used to evaluate the feasibility and applicable conditions of the anthrone-sulfuric acid method to determine the polysaccharide isolated from Bletilla striata using Dextron T20 as a standard substance.

关键词

葡聚糖T20白芨多糖星点设计-效应面法蒽酮-硫酸法含量测定

Keywords

Dextron T20Polysaccharide of Bletilla striataCentral composite designResponse surface methodologyAnthrone-sulfuric acid methodcontent determination

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