Metabolism method of dextromethorphan in vitro

ZHANG Li-li

doi:10.13406/j.cnki.cyxb.2008.03.036

您当前的位置：

首页 >

文章列表页 >

Metabolism method of dextromethorphan in vitro

更新时间：2024-01-17

- Metabolism method of dextromethorphan in vitro
- Journal of Chongqing Medical University Issue 3, Pages: 325-328(2008)
- 作者机构：
  
  1. 重庆医科大学药学院药理教研室
  2. 重庆医科大学药学院医药研究所
  3. 重庆市生物化学与分子药理重点实验室重庆市生物化学与分子药理重点实验室
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.036
  CLC： R96
- Published：2008，
扫描看全文
[1]张黎黎,刘颖菊,袁佩,杨贵忠,周岐新,廖飞.右美沙芬的体外代谢方法研究[J].重庆医科大学学报,2008(03):325-328+384.

ZHANG Li-li. Metabolism method of dextromethorphan in vitro. [J]. Journal of Chongqing Medical University (3):325-328(2008)
[1]张黎黎,刘颖菊,袁佩,杨贵忠,周岐新,廖飞.右美沙芬的体外代谢方法研究[J].重庆医科大学学报,2008(03):325-328+384. DOI： 10.13406/j.cnki.cyxb.2008.03.036.

ZHANG Li-li. Metabolism method of dextromethorphan in vitro. [J]. Journal of Chongqing Medical University (3):325-328(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.036.

摘要

目的:研究体外代谢右美沙芬的方法

并用高效液相色谱法（High performance piqid chromatography

HPLC）对右美沙芬及其产物进行测定。方法:使用正常人的肝脏匀浆在体外对右美沙芬进行代谢

用HPLC以阿霉素为内标

流动相为甲醇-10mmol/L磷酸二氢钠溶液（50:50

含0.01%庚烷磺酸钠

5%三乙胺

pH3.5）

对右美沙芬及其代谢产物进行测定。结果:在适当的pH值和温度下

加入辅酶和Mg

使用人肝脏匀浆可在体外有效代谢右美沙芬

并可以用高效液相色谱法对右美沙芬及其产物进行测定。经测定

右美沙芬平均代谢率为16.09%。结论:这种体外代谢右美沙芬的方法简便灵敏

精密度高

可用于右美沙芬的体外代谢实验。

Abstract

Objective:To investigate in vitro metabolism method and HPLC method for the dextromethorphan.Methods:Total protein of normal liver specimens metabolismed dextromethorphan in vitro.An HPLC method with fluorescence was developed to study the contents of dextromethorphan and metabolites by joining an internal standard（Adriamycin）.Methanol-10mmol/l

sodium dihydrogen phosphate solution（50:50

contain 0.01% sodium heptanesulfonate

5% triethylamine

pH 3.5）was used to the mobile phase.Results:Total protein of normal liver specimens metabolismed dextromethorphan in vitro efficiently with coenzyme

suitable temperature and pH value.Dextromethorphan and metabolites were analyed by HPLC method with fluorescence.The metabolic rate of dextromethorphan was 16.09%.Conclusion:It is suggested that the metabolism method of dextromethorphan in vitro is simple

convenient and accurate.It can be used for the determination of metabolism dextromethorphan in vitro.

关键词

右美沙芬体外代谢高效液相色谱法

Keywords

DextromethorphanIn vitro metabolismhigh performance liquid chromatography

references

Views

479

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Tanshinone on distribution of Levofloxacin in uterine tissues of pelvic inflammatory rat models

PHARMACOKINETICES OF RANITIDINE IN NORMAL SUBJECTS

Intraocular penetration of levofloxacin in rabbit eyes following topical and subconjunctival administration

Determination of vincristini sulfas in tumor cells culture media and intracellular fluid

Preparation and quality evaluation of Dextromethorphan chewing gum drug delivery system

Related Author

Qiu Feng

Zhou Yuanda

Luo Cheng

Kong Lingxi

Tang Chengyong

朱治本

严怡怡

叶运奎

Related Institution

Department of Pharmacy,the China Aviation Industrial Hospital

Department of Pharmacy,The First Affiliated Hospital of Chongqing Medical University

重庆医科大学第一医院麻醉科

重庆医科大学第一医院临床药理研究室

Dept. of Analytical Chemistry, Faculty of Lab Medicine

⁰