Effect of RPM on bcl-2 and Fas protein expression in renal ischemia-reperfusion injury of rats

ZHENG Chuan-dong

doi:10.13406/j.cnki.cyxb.2008.03.035

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Effect of RPM on bcl-2 and Fas protein expression in renal ischemia-reperfusion injury of rats

更新时间：2024-01-17

- Effect of RPM on bcl-2 and Fas protein expression in renal ischemia-reperfusion injury of rats
- Journal of Chongqing Medical University Issue 3, Pages: 285-288(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学附属第一医院血管外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.035
  CLC： R96
- Published：2008，
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[1]郑传东,苟欣,杨华安,蒲晓峰,何卫阳,武国.雷帕霉素对大鼠缺血再灌注肾Fas，bcl-2蛋白表达的影响[J].重庆医科大学学报,2008(03):285-288+293.

ZHENG Chuan-dong. Effect of RPM on bcl-2 and Fas protein expression in renal ischemia-reperfusion injury of rats. [J]. Journal of Chongqing Medical University (3):285-288(2008)
[1]郑传东,苟欣,杨华安,蒲晓峰,何卫阳,武国.雷帕霉素对大鼠缺血再灌注肾Fas，bcl-2蛋白表达的影响[J].重庆医科大学学报,2008(03):285-288+293. DOI： 10.13406/j.cnki.cyxb.2008.03.035.

ZHENG Chuan-dong. Effect of RPM on bcl-2 and Fas protein expression in renal ischemia-reperfusion injury of rats. [J]. Journal of Chongqing Medical University (3):285-288(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.035.

摘要

目的:探讨雷帕霉素（Rapamycin

RPM）对肾缺血再灌注不同时间Fas

bcl-2蛋白表达的影响。方法:Wistar大鼠54只。随机分为3组:假手术组（切除右肾

分离左肾动脉

不阻断血流

缺血前灌胃给等量生理盐水）、手术组（切除右肾

分离左肾动脉

阻断血流

缺血前灌胃给等量生理盐水）、药物组（切除右肾

分离左肾动脉

阻断血流

缺血前灌胃给RPM 4mg/（kg·d）×3d

术日术前2h灌胃

术后给药至各个观察日）。采用HE染色分析肾组织病理损伤程度

免疫组化SABC法检测bcl-2.Fas蛋白表达的变化。结果:Fas蛋白在假手术组呈阴性表达

手术组再灌注0h有少量表达

再灌注24、48及72h呈阳性表达

以48h时达高峰（P<0.05）

主要表达在皮质肾小管;药物组肾组织Fas蛋白水平显著低于手术组（P<0.05）。bcl-2蛋白在假手术组呈弱阳性表达

手术组再灌注0h有少量表达

再灌注24、48及72h呈阳性表达

48h达高峰（P<0.05）;皮质肾小管表达为主;药物组肾组织bcl-2蛋白水平显著高于手术组（P<0.05）。HE染色可见手术组肾小管上皮细胞有不同程度的片状坏死灶.肾小管中可见大量死亡脱落细胞残留物质;药物组肾组织损伤明显减轻。结论:RPM可能通过下调Fas

上调bcl-2蛋白表达水平

减轻肾缺血再灌注损伤。

Abstract

Objective:To investigate the effect of RPM on bcl-2 and Fas protein expressions at different ischemia-reperfusion points.Methods:Acute ischemic renal injury model was established(right kidney ectomized

45 min of left renal ischemia and reperfusion for Oh

24h

48h and 72h).Fifty four normal male Wistar rats were.randomly divided into 3 groups:sham-operated group(sham

without renal ischemia)

ischemia-reperfusion group(IR

without RPM)

RPM and ischemia-reperfusion group(RPM 4mg/(kg·d)×3d

reperfusion at 2h before

operation and each observation day).Pathomorphological changes of renal ischemia- reperfusion injury were observed by HE stain and SABC immunohistochemical methods were used to detect the changes of expressions of bcl-2 and Fas.Results:bcl-2 and Fas concentration were significantly higher in IR group than those in sham group(P<0.05).As compared with ischemia-reperfusion group(P<0.05)

bcl-2 expression increased and Fas expression decreased significantly in RPM+IR group.In IR group local necrosis of renal tubule epithelium cells was found by HE stain and large quantity of apoptototis residues were observed

while similar changes were scarcely visible in RPM+IR group. Conclusion:RPM has significant effect on the up-regulation of bcl-2 expression and down-regulation of Fas expression in renal ischemia-reperfusion injury to achieve the purpose of alleviating the injury.

关键词

再灌注损伤雷帕霉素凋亡FasBcl-2

Keywords

reperfusion injuryrapamycinapoptosisFasBcl-2

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