The effect of resveratrol combined with arabinosylcytosin in K562 cell Fas/FasL and survivin expression

CHEN Jian-bin

doi:10.13406/j.cnki.cyxb.2008.03.024

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The effect of resveratrol combined with arabinosylcytosin in K562 cell Fas/FasL and survivin expression

更新时间：2024-01-17

- The effect of resveratrol combined with arabinosylcytosin in K562 cell Fas/FasL and survivin expression
- Journal of Chongqing Medical University Issue 3, Pages: 308-311(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院血液科
  2. 重庆医科大学附属第一医院血液科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.024
  CLC： R733.7
- Published：2008，
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[1]陈建斌,鲁敏,杨泽松,黄宗干.白藜芦醇联合阿糖胞苷对人自血病K562细胞Fas/FasL、survivin表达影响[J].重庆医科大学学报,2008(03):308-311.

CHEN Jian-bin. The effect of resveratrol combined with arabinosylcytosin in K562 cell Fas/FasL and survivin expression. [J]. Journal of Chongqing Medical University (3):308-311(2008)
[1]陈建斌,鲁敏,杨泽松,黄宗干.白藜芦醇联合阿糖胞苷对人自血病K562细胞Fas/FasL、survivin表达影响[J].重庆医科大学学报,2008(03):308-311. DOI： 10.13406/j.cnki.cyxb.2008.03.024.

CHEN Jian-bin. The effect of resveratrol combined with arabinosylcytosin in K562 cell Fas/FasL and survivin expression. [J]. Journal of Chongqing Medical University (3):308-311(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.024.

摘要

目的:探讨白藜芦醇及其联合阿糖胞苷对K562细胞凋亡的影响及相关机制。方法:采用免疫组织化学法和RT-PCR法分别检测白藜芦醇及其联合阿糖胞苷处理K562细胞48h后Fas、FasL和Survivin表达情况。结果:K562细胞分别经白藜芦醇、阿糖胞苷处理48h后白藜芦醇组和阿糖胞苷组Fas表达光密度值较对照组升高（P<0.05）

FasL表达上调较对照组有显著性变化（P<0.05）

两药联合作用Fas表达较单用白藜芦醇无显著性变化（P>0.05）

而FasL表达上调较单用白藜芦醇或阿糖胞苷有显著性变化（P<0.05）。白藜芦醇、阿糖胞苷单独及联合作用48h后

K562细胞的Survivin mRNA表达较细胞对照组下降（P<0.05、P<0.01）.两药联合作用Survivin mRNA表达较单用白藜芦醇无显著性变化（P>0.05）。结论:白藜芦醇单独及联合阿糖胞苷能通过上调Fas/FasL的表达

激活Fas/FasL凋亡途径

诱导人白血病K562细胞凋亡

同时下调Survivin的表达促进凋亡的发生。

Abstract

Objective:This study was aimed to explore the apoptotic effect the K562 cells treated by resveratrol and/or arabinosylcytosin.Methods:Fas/FasL expressian was detected by immunohistochemical method and survivin expression was detected by RT-PCR in K562 cells

48 hours after treatment by resveratrol and arabinosylcytosin respectively.Results: Fas/FasL expression in K562 cells

treated by resveratrol and arabinosylcytosin respectively

was significantly increased compared with the control group(P<0.05).However

the effect of resveratrol combined with arabinosylcytosin in K562 cell Fas expression showed no significance changes in comparison with resveratrol or arabinosylcytosin(P>0.05)

contrary to FasL expression(P<0.05).Survivin mRNA expression was decreased after 48 hours in K562 cells treated by resveratrol and/or arabinosylcytosin in comparison with the control group(P<0.05

P<0.01).However

the effect of resveratrol combined with arabinosylcytosin in K562 cell Survivin mRNA expression showed no significance changes in comparison with resveratrol or arabinosylcytosin(P>0.05).Conclusions:In conclusion the resveratrol and/or arabinosylcytosin could upregulate Fas/FasL expression and activate the apoptosis pathway of Fas/FasL to induce the apoptosis of K562 cells.It could downregulate the expression of survivin to promote apoptosis.The two drugs had synergistic effect.

关键词

白藜芦醇阿糖胞苷K562Fas/FaslSurvivin

Keywords

resveratrolArabinosylcytosinK562Fas/FaslSurvivin

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