Molecular mechanism of proliferation and apoptosis in breast cancer MCF-7 cells by penta-brominated diphenyl ethers

LUO Dong

doi:10.13406/j.cnki.cyxb.2008.03.020

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Molecular mechanism of proliferation and apoptosis in breast cancer MCF-7 cells by penta-brominated diphenyl ethers

更新时间：2024-01-17

- Molecular mechanism of proliferation and apoptosis in breast cancer MCF-7 cells by penta-brominated diphenyl ethers
- Journal of Chongqing Medical University Issue 3, Pages: 270-273(2008)
- 作者机构：
  
  1. 重庆医科大学公共卫生学院环境卫生教研室
  2. 重庆医科大学公共卫生学院环境卫生教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.020
  CLC： R737.9
- Published：2008，
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[1]罗东,詹平.五溴联苯醚影响乳腺癌MCF-7细胞增殖和凋亡的机制探讨[J].重庆医科大学学报,2008(03):270-273.

LUO Dong. Molecular mechanism of proliferation and apoptosis in breast cancer MCF-7 cells by penta-brominated diphenyl ethers. [J]. Journal of Chongqing Medical University (3):270-273(2008)
[1]罗东,詹平.五溴联苯醚影响乳腺癌MCF-7细胞增殖和凋亡的机制探讨[J].重庆医科大学学报,2008(03):270-273. DOI： 10.13406/j.cnki.cyxb.2008.03.020.

LUO Dong. Molecular mechanism of proliferation and apoptosis in breast cancer MCF-7 cells by penta-brominated diphenyl ethers. [J]. Journal of Chongqing Medical University (3):270-273(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.020.

摘要

目的:观察环境化合物五溴联苯醚（Penta-brominated diphenyl ethers

5-BDE）影响人乳腺癌细胞MCF-7增殖和凋亡的分子生物学作用机制。方法:将MCF-7细胞在DMEM培养液中进行常规传代培养。采用MTT比色法观察5-BDE对MCF-7细胞增殖的影响

用逆转录聚合酶链反应（RT-PCR）技术和免疫组化方法检测5-BDE对Bcl-2和Bax基因表达的影响。结果:与溶剂对照组相比

在1×(10

-8

～10

-4

)mol/L浓度范围内

5-BDE与1×10

-8

mol/L雌二醇作用类似

可显著促进MCF-7细胞增殖（P

0.05）

并表现出良好的剂量-效应和时间-效应关系。RT-PCR及免疫组化结果显示

5-BDE可促进MCF-7细胞Bcl-2和抑制Bax蛋白表达。结论:5-BDE可通过影响细胞增殖和凋亡相关调节基因Bcl-2和Bax表达而调节人乳腺癌细胞MCF-7的增殖和凋亡作用。

Abstract

Objective:To investigate the molecular mechanisms of proliferation and apoptosis by penta-brominated Diphenyl Ethers through regulation of mRNA and protein expression of Bcl-2 and Bax in breast cancer MCF-7 cells.Methods:Human breast cancer MCF-7 cells were grown in DMEM medium

MTT assay was employed to investigate cell proliferation.RT-PCR and immunohistochemistry was performed to determine mRNA and protein expression of Bcl-2 and Bax

Results:Within the range of 1×(10

-8

～10

-4

)mol/L

5-BDEs stimulated proliferation in MCF-7 cells with a dose-dependent and time-dependent fashion.RT-PCR and immunohistochemistry analyses revealed that treatment with 1×(10

-8

～10

-4

)mol/L for 72h resulted in increased mRNA and protein expression of Bcl-2 and inhibition of Bax.Conclusion:5-BDE possessed estrogenic activity and could markedly stimulate proliferation in estrogen responsive breast cancer MCF-7 cells.Up-regulation of Bcl-2 mRNA and protein expression was suggested to be involved in this biological effect.

关键词

五溴联苯醚MCF-7细胞Bcl-2Bax

Keywords

5-BDEMCF-7 cellBcl-2Bax

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