Preparation and characterization of monoclonal antibody against S protein of HBV

WANG Xue-mei

doi:10.13406/j.cnki.cyxb.2008.03.006

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Preparation and characterization of monoclonal antibody against S protein of HBV

更新时间：2024-01-17

- Preparation and characterization of monoclonal antibody against S protein of HBV
- Journal of Chongqing Medical University Issue 3, Pages: 267-269(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院病毒性肝炎研究所感染性疾病分子生物学教育部重点实验室
  2. 第三军医大学附属西南医院肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.006
  CLC： R392.11
- Published：2008，
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[1]王雪梅,赖梅梅,张晓丽,张祯祯,陈婧,张君,黄爱龙.抗HBsAg单克隆抗体的制备与生物学特性的鉴定[J].重庆医科大学学报,2008(03):267-269.

WANG Xue-mei. Preparation and characterization of monoclonal antibody against S protein of HBV. [J]. Journal of Chongqing Medical University (3):267-269(2008)
[1]王雪梅,赖梅梅,张晓丽,张祯祯,陈婧,张君,黄爱龙.抗HBsAg单克隆抗体的制备与生物学特性的鉴定[J].重庆医科大学学报,2008(03):267-269. DOI： 10.13406/j.cnki.cyxb.2008.03.006.

WANG Xue-mei. Preparation and characterization of monoclonal antibody against S protein of HBV. [J]. Journal of Chongqing Medical University (3):267-269(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.006.

摘要

目的:制备分泌抗HBV S蛋白的单克隆抗体的杂交瘤细胞株

并鉴定其特性

为建立快速诊断HBV感染的方法奠定基础。方法:以重组乙肝疫苗免疫BALB/c小鼠

通过细胞融合建立了能稳定分泌抗HBsAg单抗的杂交瘤细胞株

利用间接ELISA技术和Western blot进行单抗特异性的鉴定

同时采用间接ELISA方法鉴定mAb的Ig亚类

检测mAb的效价及相对亲和力。结果:获得1株可分泌特异性mAb的杂交瘤细胞4D2

其抗体亚类为IgG

型

腹水效价为1:10

相对亲和力在10

以上。结论:成功地制备出抗HBV s蛋白的单克隆抗体

为建立快速特异检测HBV感染的实验方法提供了有力的工具。

Abstract

Objective:To prepare anti-HBV S protein hybridoma cell line

this will be helpful to the establishment of rapid HBV infection diagnosis assay.Methods:BALB/c mice were immunized with the recombinant Hepatitis B vaccine.A hybridoma cell line which consistently secreted monoclonal antibody（McAb）against HBsAg was obtained through cell fusion.The specificity of the McAb was analysed by indirect ELISA and Western blot analysis.The immunoglobulin（Ig）subtypethe ascites titers

and the affinity of the obtained mAbs were determined by indirect ELISA.Results:From over hundred positive hybridomas which secreted anti-S protein mAbs

one of hybridomas was screened out

designated 4D2.The subtype of the McAb was lgG

. The titer of 4D2 mAb produced by ascites fluid was over 1:10

.The relative affinity constant of 4D2 mAb was determined as 10

.Conclusion:One of high titer

specific mAb against HBV S protein has been successfully prepared and primarily identified

which may be useful in the development of a rapid and convenient diagnostic kit for detection of HBV infection.

关键词

乙型肝炎病毒乙型肝炎表面抗原单克隆抗体

Keywords

hepatitis B virusHepatitis B surface antigen(HBsAg)monoclonal antibody

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