Effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in chick chorioallantoic membrane

WANG Jing

doi:10.13406/j.cnki.cyxb.2008.03.004

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Effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in chick chorioallantoic membrane

更新时间：2024-01-17

- Effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in chick chorioallantoic membrane
- Journal of Chongqing Medical University Issue 3, Pages: 261-263(2008)
- 作者机构：
  
  1. 重庆医科大学超声影像学研究所,重庆医科大学超声影像学研究所,重庆医科大学超声影像学研究所,重庆医科大学超声影像学研究所,重庆医科大学附属第二医院超声科,重庆医科大学附属第二医院超声科,重庆,400010
  2. ,重庆,400010
  3. 400010 重庆医科大学附属第二医院康复医学中心,重庆 400010,重庆 400010,重庆 400010,重庆,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.004
  CLC： R363
- Published：2008，
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[1]王静,王志刚,许川山,李攀,张群霞,张茂惠.超声激活血卟啉声动力作用对鸡胚绒毛尿囊膜VEGF表达的影响[J].重庆医科大学学报,2008(03):261-263+273.

WANG Jing. Effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in chick chorioallantoic membrane. [J]. Journal of Chongqing Medical University (3):261-263(2008)
[1]王静,王志刚,许川山,李攀,张群霞,张茂惠.超声激活血卟啉声动力作用对鸡胚绒毛尿囊膜VEGF表达的影响[J].重庆医科大学学报,2008(03):261-263+273. DOI： 10.13406/j.cnki.cyxb.2008.03.004.

WANG Jing. Effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in chick chorioallantoic membrane. [J]. Journal of Chongqing Medical University (3):261-263(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.004.

摘要

目的:本研究探讨超声激活血卟啉（Hematoporphrin

Hp）声动力作用对鸡胚绒毛尿囊膜（Chorioallantoie membrane

CAM）的血管内皮细胞生长因子（Vascular endothelial cell growth factor

VEGF）表达的影响

为应用声动力疗法（Sonodynamic therapy

SDT）进行抗肿瘤血管新生提供实验基础。方法:种蛋随机分为4组

孵育7d后建CAM模型

随机分为空白组、单纯超声组、单纯Hp组、声动力治疗组。各组药物分别加于CAM表面的载体上

作用后制备标本

用免疫组化检测各组鸡胚CAM VEGF表达情况。结果:与空白组相比

单纯Hp组差异无统计学意义（P>0.05）;单纯超声组、声动力治疗组与之差异有统计学意义（P<0.05）;单纯超声组与声动力治疗组之间比较

差异有统计学意义（P<0.05）。结论:Hp介导的声动力作用显著抑制了鸡胚绒毛尿囊膜VEGF的表达

这可能是其抑制新生血管形成的重要机制。

Abstract

Objective:To investigate the effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in Chick Chorioallantoic Membrane and provide experimental basis for appling sonodynamic therapy(SDT)on anti-angiogenesis in tumor therapy.Methods:The hatching eggs cultured for 7 days were randomly divided into 4 groups:blank control group

ultrasound group

hematoporphrin group

sonodynamic therapy group.The express of VEGF in CAM was detected by immunity histochemistry.Result:There were significant differences between control group and ultrasound group(P<0.05)and also between control group and SDT group(P<0.05)

and between ultrasound group and SDT group(P<0.05);while no difference between control group and hematoporphfin group(P>0.05)

conclusion The results show that sonodynamic therapy with hematoporphrin has a significant inhibitionary effect on the express of VEGF in CAM and this maybe is the most important mechanism of its anti-angiogenesis effect.

关键词

声动力作用血卟啉血管生成绒毛尿囊膜血管内皮细胞生长因子

Keywords

Sonodynamie effectHematoporphrinChorioallantoie membraneAngiogenesisVEGF

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Department of Orthopaedics, College of Clinical Medicine , Chongqing Medical University

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