Effect of angelica polysaccharides on adhesion molecules expressions in murine hematopoietic cells

DU Xiao-li

doi:10.13406/j.cnki.cyxb.2008.03.003

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Effect of angelica polysaccharides on adhesion molecules expressions in murine hematopoietic cells

更新时间：2024-01-17

- Effect of angelica polysaccharides on adhesion molecules expressions in murine hematopoietic cells
- Journal of Chongqing Medical University Issue 3, Pages: 274-276(2008)
- 作者机构：
  
  1. 重庆医科大学基础医学院组胚教研室
  2. 重庆医科大学基础医学院组胚教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2008.03.003
  CLC： R285.5
- Published：2008，
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[1]杜小丽,王改琴,吴宏.当归多糖对小鼠造血细胞表面粘附分子表达的影响[J].重庆医科大学学报,2008(03):274-276+300.

DU Xiao-li. Effect of angelica polysaccharides on adhesion molecules expressions in murine hematopoietic cells. [J]. Journal of Chongqing Medical University (3):274-276(2008)
[1]杜小丽,王改琴,吴宏.当归多糖对小鼠造血细胞表面粘附分子表达的影响[J].重庆医科大学学报,2008(03):274-276+300. DOI： 10.13406/j.cnki.cyxb.2008.03.003.

DU Xiao-li. Effect of angelica polysaccharides on adhesion molecules expressions in murine hematopoietic cells. [J]. Journal of Chongqing Medical University (3):274-276(2008) DOI： 10.13406/j.cnki.cyxb.2008.03.003.

摘要

目的:探讨当归多糖（Angelica polysaccharides

APS）对小鼠造血细胞表面粘附分子表达的影响

旨在为阐述APS在造血调控和动员方面的机制提供实验依据。方法:采用外周血WBC、骨髓单个核细胞（Marraw mononuclear cell

MNC）计数

流式细胞术检测外周血和骨髓Sea-1

细胞率以及骨髓MNC和Sca-1

细胞表面CD49d（VLA-4的α链）、CD44表达情况。结果:1采用4mg/kg APS动员后小鼠外周血的WBC、Sca-1

细胞数明显高于NS组（P

0.01）

而骨髓MNC和Sca-1

细胞数却明显低于NS组（P

0.01）;2APS组骨髓MNC及Sca-1

细胞表面CD49d表达明显下降（P

0.05）;骨髓MNC表面CD44表达阳性率下降（P

0.05）

但荧光强度无明显变化;骨髓Sca-1

细胞表面CD44的表达与NS组无显著性差异。结论:APS可能通过降低造血细胞表面的某些粘附分子的表达而产生动员效果。

Abstract

Objective:To explore the effect of angelica polysaccharide(APS)on adhesion molecules expressions in murine hematopoietic cells

in order to provide experimental evidence of APS on hematopoietic regulation and the mechanism of mobilizing.Methods:Techniques of peripheral blood white blood cell(WBC)and bone marrow mononuclear cells(MNC)count were made.Flow cytometry detected the rate of Sca-1~+ cells in peripheral blood and bone marrow and the expression of CD49d (VLA-4 of a chain)

CD44 on bone marrow MNC and Sca-1~+ cells.Results:1.In APS group(4mg/kg):the number of peripheral blood WBC and Sca-1~+ cells were markedly higher than that of NS group(P<0.01)

and bone marrow MNC and Sca-1~+ cells was significantly lower than that of NS group(P<0.01);2.In APS group:the expression of CD49d on bone marrow MNC and Sca-1~+ cells significantly declined(P<0.05);the percent of CD44’expression on bone marrow MNC also decreased(P<0.05)

but we did not observe any change of the mean fluorescence intensity at the same time

or that of CD44’expression on bone marrow Sca-1~+ cells.Conclusion:APS may reduce adhesion molecules expressions on some hematopoietic cells

resulting in mobilizing hematopoietic stem and progenitor cells cells(HSPC)from the bone marrow into the peripheral blood.

关键词

当归多糖造血干/祖细胞粘附分子VLA-4CD44

Keywords

angelica polysaccharidesHematopoietic stem and progenitor cellsadhesion moleculeVLA-4CD44

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