Establishment of a two-dimensional electrophoresis technology on colon carcinoma

MA Guozhong; et al

doi:10.13406/j.cnki.cyxb.2007.12.023

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Establishment of a two-dimensional electrophoresis technology on colon carcinoma

更新时间：2024-01-17

- Establishment of a two-dimensional electrophoresis technology on colon carcinoma
- Journal of Chongqing Medical University Issue 12, Pages: 1268-1271(2007)
- 作者机构：
  
  1. 四川绵阳市第三人民医院普通外科
  2. 重庆医科大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.12.023
  CLC： R735.35
- Published：2007，
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[1]马国中,王继生,周成林.结肠癌蛋白质双向电泳技术的建立[J].重庆医科大学学报,2007(12):1268-1271+1310.

MA Guozhong, et al. Establishment of a two-dimensional electrophoresis technology on colon carcinoma. [J]. Journal of Chongqing Medical University (12):1268-1271(2007)
[1]马国中,王继生,周成林.结肠癌蛋白质双向电泳技术的建立[J].重庆医科大学学报,2007(12):1268-1271+1310. DOI： 10.13406/j.cnki.cyxb.2007.12.023.

MA Guozhong, et al. Establishment of a two-dimensional electrophoresis technology on colon carcinoma. [J]. Journal of Chongqing Medical University (12):1268-1271(2007) DOI： 10.13406/j.cnki.cyxb.2007.12.023.

摘要

目的:建立结肠癌双向电泳技术。方法:利用不同体积的裂斛液提取结肠癌中的蛋白质

并通过不同的蛋白质上样量（1mg

2mg

3mg）

不同样品制备方式

进行双向电泳

考马斯亮蓝染色

图谱分析。结果:在等电点3～10

分子量6.5～200ku范围内分离得到蛋白质斑点为

1mg蛋白质上样量为658个蛋白质斑点

2mg为820个

3mg为845个斑点。2mg蛋白质上样量的双向电泳图谱更清晰

分离更好。制备方式2可以满足双相电泳的需要。结论:成功建立了结肠癌蛋白质的双向电泳技术。

Abstract

Objective:To establish a two-dimensional electrophoresis technology tissue protein of colon carcinnma. Methods: Lysis buffer of different volume was taken to extract tissue proteins of colon carcinoma

and different protein quantities (1mg

2mg

3mg) different preparation ways were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein

and patterns were analyzed. Results:With a molecular mass between 6.5～200ku and isoelectric points (pI) from 3～10

1mg proteins obtained 569 protein spots

2mg 786

and 3mg 835. Pattern of 2mg protein and the sample two were the best. Conclusion:A two-dimensional electrophoresis technology on tissue protein of colon carcinoma has been established successfully.

关键词

蛋白质组学双向电泳结肠癌

Keywords

proteomicsTwo-dimensional electrophoresiscolon carcinoma

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