Establishment of proteomic techniques and two-dimensional electrophoregram of human hepatocellular carcinoma cell line QGY-7701

YUAN Yonghua; et al

doi:10.13406/j.cnki.cyxb.2007.12.022

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Establishment of proteomic techniques and two-dimensional electrophoregram of human hepatocellular carcinoma cell line QGY-7701

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- Establishment of proteomic techniques and two-dimensional electrophoregram of human hepatocellular carcinoma cell line QGY-7701
- Journal of Chongqing Medical University Issue 12, Pages: 1233-1236(2007)
- 作者机构：
  
  1. 重庆医科大学药学院药物化学教研室
  2. 重庆医科大学病理生理学教研室
  3. 重庆医科大学基础医学研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.12.022
  CLC： R735.7
- Published：2007，
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[1]袁拥华,李伟,杨晓兰,李龙江,汤为学,黄开顺,余瑜.人肝癌细胞株QGY-7701蛋白质组学研究技术及二维电泳图谱的建立[J].重庆医科大学学报,2007(12):1233-1236.

YUAN Yonghua, et al. Establishment of proteomic techniques and two-dimensional electrophoregram of human hepatocellular carcinoma cell line QGY-7701. [J]. Journal of Chongqing Medical University (12):1233-1236(2007)
[1]袁拥华,李伟,杨晓兰,李龙江,汤为学,黄开顺,余瑜.人肝癌细胞株QGY-7701蛋白质组学研究技术及二维电泳图谱的建立[J].重庆医科大学学报,2007(12):1233-1236. DOI： 10.13406/j.cnki.cyxb.2007.12.022.

YUAN Yonghua, et al. Establishment of proteomic techniques and two-dimensional electrophoregram of human hepatocellular carcinoma cell line QGY-7701. [J]. Journal of Chongqing Medical University (12):1233-1236(2007) DOI： 10.13406/j.cnki.cyxb.2007.12.022.

摘要

目的:建立人肝癌细胞株QGY-7701蛋白质组学研究技术。方法:利用两种不同的裂解液提取细胞蛋白

进行双向电泳

经PDQuest软件分析与比较后

挑选匹配良好的10个蛋白点

进行基质辅助激光解析/电离-飞行时间质谱（MALDI-TOF-MS）的鉴定。结果:建立了稳定的人肝癌细胞株QGY-7701的双向电泳图谱

裂解液Ⅱ比裂解液Ⅰ提取的蛋白质量多25%

2-DE胶上蛋白点数多32%

从10个候选蛋向中鉴定出9个有意义的蛋白（P<0.05）。结论:初步建立了人肝癌细胞株QGY-7701的蛋白质组学研究技术

为进一步开展药物蛋白质组学研究奠定基础。

Abstract

Objective:To establish proteomic technique system of human hepatocellular carcinoma cell line QCY-7701. Method:Two different lysis buffer were taken to extract cell proteins. After two-dimensional gel electrophuresis (2-DE) and PDQuest analysis

10 good-matched protein spots were chosen to be identified by MALDI-TOF-MS. Results:A steady 2-DE electrophregram of hepatocellular carcinoma cell line QGY-7701 was established. Compared with lysis bufferⅠ

protein quantities extracted from lysis bufferⅡincreased by 25%;protein spots in 2-DE gel increased by 32%. 9 out of 10 candidate protein spots were successfully identified (P<0.05). Conclusions:Our established proteomic technique of human hepatocellular carcinoma cell line OGY-7701 can be effectively applied in cumparative pruteomics of drugs.

关键词

人肝癌细胞株QGY-7701蛋白质组学双向电泳

Keywords

Human hepatocellular carcinoma cell line QGY-7701proteomics2-dimensional gel electrophoresis(2-DE)

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