In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion

LIU Xiru; et al

doi:10.13406/j.cnki.cyxb.2007.12.014

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In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion

更新时间：2024-01-17

- In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion
- Journal of Chongqing Medical University Issue 12, Pages: 1265-1267(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院辅助生殖中心
  2. 四川大学华西第二医院生殖中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.12.014
  CLC： R714.21
- Published：2007，
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[1]刘西茹,李尚为,汪燕,樊伟.CTLA-4抗体诱导原因不明复发性流产患者Th1／Th2转换的体外研究[J].重庆医科大学学报,2007(12):1265-1267+1307.

LIU Xiru, et al. In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion. [J]. Journal of Chongqing Medical University (12):1265-1267(2007)
[1]刘西茹,李尚为,汪燕,樊伟.CTLA-4抗体诱导原因不明复发性流产患者Th1／Th2转换的体外研究[J].重庆医科大学学报,2007(12):1265-1267+1307. DOI： 10.13406/j.cnki.cyxb.2007.12.014.

LIU Xiru, et al. In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion. [J]. Journal of Chongqing Medical University (12):1265-1267(2007) DOI： 10.13406/j.cnki.cyxb.2007.12.014.

摘要

目的:观察CTLA-4抗体在诱导原因不明复发性流产患者Th1/Th2转换中的作用。方法:用人绒毛膜癌细胞株JEG-3制备的滋养细胞抗原刺激30例复发性流产患者外周血单核细胞

实验组加CTLA-4抗体（10μg/ml组和1μg/ml组）

对照组加IgG（10μg/ml组和1μg/ml组）培养

分别取24h、72h上清液

ELISA法测其IL-2、IFN-γ和IL-4的水平。结果:①与同浓度对照组相比

实验组产生的IL-4均显著降低（P<0.01）

而产生的IFN-γ、IL-2均明显升高（P<0.05）。②与CTLA-4抗体1μ/ml组相比

CTLA-4抗体10μg/ml组产生的IL-4稍低

而IFN-γ和IL-2稍高

但无统计学差异（P>0.05）

结论:CTLA-4抗体可以上调Th1型细胞因子

下调Th2型细胞因子

提示CTLA-4抗体体外阻断CTLA-4可诱导原因不明复发性流产的Th1/Th2失衡更向Th1极化

不利于妊娠。

Abstract

Objective:To investigate the effects of anti-CTLA4 antibody on Th1/Th2 shift in unexplained recurrent spontaneous abortion. Methods:The levels of IL-2

IFN-γand IL-4 were assessed by ELISA in the supernatant of cultured maternal peripheral blood mnnonuclear cells(PBMC) from 30 patients of Unexplained RSA. Cytokine synthesis was induced by activation with antigens from a chnriocarcinoma cell line of JEG-3

with anti-CTLA4 antibodv or IgG. Results:We detected significantly greater levels of the Th1 cytokines IL-2、IFN-γ(P<0.05)and lower levels of the Th2 cytnkines IL-4(P<0.01)in anti-CTLA4 antibody groups

compared with control group of the same concentration. Between the trial groups of different concentration

there is no significance(P>0.05). Conclusion:Anti-CTLA4 antibody can up-regulate Th1-type cytokines and down-regulate Th2-type cytokines

which indicates that in URSA anti-CTLA4 antibody could swith T cell responses to Thl

and is harmful to URSA.

关键词

流产复发性Th1细胞Th2细胞CTLA-4

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