Study on the biocompatibility of semiconductor quantum dots to the rat dental papillae cells (RDPC) cultured in vitro

SUN Deping; et al

doi:10.13406/j.cnki.cyxb.2007.12.001

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Study on the biocompatibility of semiconductor quantum dots to the rat dental papillae cells (RDPC) cultured in vitro

更新时间：2024-01-17

- Study on the biocompatibility of semiconductor quantum dots to the rat dental papillae cells (RDPC) cultured in vitro
- Journal of Chongqing Medical University Issue 12, Pages: 1237-1241(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院口腔颌面外科
  2. 重庆医科大学附属第一医院口腔颌面外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.12.001
  CLC： R329
- Published：2007，
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[1]孙德平,杨凯,陈睿.半导体量子点对原代培养大鼠牙乳头细胞生物相容性的研究[J].重庆医科大学学报,2007(12):1237-1241.

SUN Deping, et al. Study on the biocompatibility of semiconductor quantum dots to the rat dental papillae cells (RDPC) cultured in vitro. [J]. Journal of Chongqing Medical University (12):1237-1241(2007)
[1]孙德平,杨凯,陈睿.半导体量子点对原代培养大鼠牙乳头细胞生物相容性的研究[J].重庆医科大学学报,2007(12):1237-1241. DOI： 10.13406/j.cnki.cyxb.2007.12.001.

SUN Deping, et al. Study on the biocompatibility of semiconductor quantum dots to the rat dental papillae cells (RDPC) cultured in vitro. [J]. Journal of Chongqing Medical University (12):1237-1241(2007) DOI： 10.13406/j.cnki.cyxb.2007.12.001.

摘要

目的:研究半导体量子点（QDs）对原代培养的大鼠牙乳头细胞（Rat dental papillae cells

RDPCs）的生物相容性。方法:①选择刚出生3天的新生SD大鼠

取出磨牙牙胚

分离牙乳头

用酶消化法培养SD大鼠牙乳头细胞

用波形丝蛋白和细胞角蛋白免疫化学染色鉴定细胞来源

观察细胞的生长规律;②把605QDs、525QDs及两种量子点等浓度的混合物按3种不同浓度与RDPCs共同培养

观察QDs对RDPCs生长和形态的影响;③用MTT法检测不同浓度的605QDs、525QDs及两种量子点等浓度的混合物对RDPCs的毒性作用结果:①:体外培养的RDPCs生长良好

波形丝蛋白染色阳性而细胞角蛋白染色阴性。②不同浓度的605QDs、525QDs及两种量子点的等浓度混合物对体外培养的RDPCs没有毒性作用

对其生长和形态也没有影响。结论:605QDs、525QDs及两种量子点等浓度的混合物在一定的浓度范围内时RPDCs具有良好的生物相容性

为605QDs、525QDs在活体生理条件下用于活细胞内的多通道信号研究提供了科学依据。

Abstract

Objective:To study the biocompatibility of semiconductor quantum dots to the rat dental papillae cells(RDPCs) cultured in vitro. Methods:①RDPCs isolated and cultured with enzyme digestion. Wave form fibroin and cytokeratin were used to identify the cells resource and the growth rules of cells was observed.②To study the effeel of QDs to the RDPCs

The RDPCs were cultured with the 605QDs

525QDs and those mixture in different density.③With the MTT

to studv the cytotoxicity of QDs to the RDPCs

The RDPCs were cultured with the 605QDs

525QDs and those mixture in different density. Results:①RDPCs cultured in vitro were well growing. Wave form fibroin staining was positive and cytokeratin staining was negative in RDPCs.②The 605QDs

525QDs and those mixture in different density show no negative effect and cytotoxicity on the growth of the RDPCs. Conclusion:The 6050Ds、525QDs in a range of density have well biocmpatibility to the live cells

which supplied scientific evidence for the research of multichannel signal in living cells in vivo physiological condition

关键词

半导体量子点(半导体纳米微晶体)大鼠牙乳头细胞生物相容性口腔活细胞

Keywords

Semiconductor quantum dots (semiconductor nanoerystals)Rat dental papillae cells(RDPCs)biocompatibilityoral cavityLive cells

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Related Institution

Department of Orthopedics,The First Affiliated Hospital of Chongqing Medical University

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四川大学纳米生物材料研究中心

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