Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis

LI Wengui

doi:10.13406/j.cnki.cyxb.2007.07.002

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Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis

更新时间：2024-01-17

- Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis
- Journal of Chongqing Medical University Issue 7, Pages: 676-678(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院传染病寄生虫病研究所
  2. 重庆医科大学附属第一医院传染病寄生虫病研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.07.002
  CLC： R392
- Published：2007，
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[1]李文桂,王鸿,朱佑明.多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞亚群的动态观察[J].重庆医科大学学报,2007(07):676-678.

LI Wengui. Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis. [J]. Journal of Chongqing Medical University (7):676-678(2007)
[1]李文桂,王鸿,朱佑明.多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞亚群的动态观察[J].重庆医科大学学报,2007(07):676-678. DOI： 10.13406/j.cnki.cyxb.2007.07.002.

LI Wengui. Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis. [J]. Journal of Chongqing Medical University (7):676-678(2007) DOI： 10.13406/j.cnki.cyxb.2007.07.002.

摘要

目的:动态观察多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞亚群的变化。方法:将疫苗采用鼻腔内接种免疫BALB/C鼠

在免疫后0、2、4、6、8、10、12、14、16和18周各剖杀4只小鼠取脾

分离脾细胞

流式细胞仪检测脾CD

和CD

T淋巴细胞亚群的百分比

同时设有PBS对照。结果:疫苗接种组的脾CD

和CD

T细胞亚群分别在免疫后2～12周和2～18周升高

分别在免疫后6和10周达最高水平。结论:CD

T细胞亚群数目在多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫早期（2～6周）显著增加

Abstract

Objective:To dynamically observe changes of subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis（Em）.Methods:BALB/C mice were intranasally vacci- nated with the vaccine and killed to get spleen at 0

16 and 18w of immunization respectively.Splenocytes were.separated to measure subsets of CD

and CD

T cells by FACsort

PBS served as control.Results:In the groups of im- munization

and CD

subsets increased obviously at 2～12w and 2-18w respectively

which reached the highest level at 6 and 10w respectively.Conclusion:The number of CD

subsets of splenocytes increases remarkably in the early stage （2～6week）by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine.

关键词

多房棘球绦虫重组BCG-EmⅡ/3疫苗重组BCG-Em 14-3-3疫苗脾细胞亚群

Keywords

Echinococcus multilocularisRecombinant BCG-EmⅡ/3 vaccineRecombinant BCG-Em14-3-3 vaccineSubsets of splenocytes

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Related Institution

Institute of Infectious and Parasitic Disease,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第一医院传染病寄生虫病研究所重庆市传染病寄生虫病重点实验室

Institute of Infectious and Parasitic Diseases,Chongqing Medical University

重庆医科大学传染病寄生虫病研究所

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