Expression of aquaporin-4 in rat pancreas

WANG Kejian

doi:10.13406/j.cnki.cyxb.2007.07.001

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Expression of aquaporin-4 in rat pancreas

更新时间：2024-01-17

- Expression of aquaporin-4 in rat pancreas
- Journal of Chongqing Medical University Issue 7, Pages: 673-675(2007)
- 作者机构：
  
  1. 重庆医科大学基础医学院神经生物学实验室
  2. 重庆医科大学基础医学院神经生物学实验室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.07.001
  CLC： R341
- Published：2007，
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[1]汪克建,孙善全,冉建华,陈海,谭戈,杨美.水通道蛋白-4在胰腺的表达[J].重庆医科大学学报,2007(07):673-675+745.

WANG Kejian. Expression of aquaporin-4 in rat pancreas. [J]. Journal of Chongqing Medical University (7):673-675(2007)
[1]汪克建,孙善全,冉建华,陈海,谭戈,杨美.水通道蛋白-4在胰腺的表达[J].重庆医科大学学报,2007(07):673-675+745. DOI： 10.13406/j.cnki.cyxb.2007.07.001.

WANG Kejian. Expression of aquaporin-4 in rat pancreas. [J]. Journal of Chongqing Medical University (7):673-675(2007) DOI： 10.13406/j.cnki.cyxb.2007.07.001.

摘要

目的:观察水通道蛋白-4（AQP4）在大鼠胰腺的表达和分布特点

为研究胰腺的分泌及调节机制提供形态学基础。方法:利用免疫组化和原位杂交技术

检测AQP4及其mRNA在胰腺外分泌部和内分泌部的表达情况。结果:免疫组化显示

AQP4在胰腺外分泌部腺泡细胞膜上表达较弱

而在胰岛内分泌部细胞有明显的表达

主要位于细胞膜

呈小泡状;原位杂交显示

AQP4mRNA在外分泌部腺泡细胞有较弱的表达

在胰岛的内分泌细胞胞浆中有明显的表达。结论:根据AQP4及其mRNA在胰腺的表达和分布特点

提示其参与了胰岛内分泌细胞合成和分泌激素过程中水和电解质平衡的精细调节;而AQP4可能对胰腺外分泌过程的调节作用较小。

Abstract

Objective:To define the localization of aquaporin-4(AQP4)and its mRNA expression in the pancreas.So as to pro- vide morphological basis for the secretory function and regulation mechanisms in the pancreas.Methods:Immunohistochemical (IHC)and in situ hybridization(ISH)staining techniques were used to evaluate the expression of AQP4 and mRNA expression in pancreas.Results:IHC showed that AQP4 and its mRNA were expressed in the membrane of pancreas islet cells;however light-coloured was expressed in the acinus cell.Conclusion:AQP4 might contribute to the precise regulation of water and ion balance in pancreas islet cells

which may be fluctuated during the process of synthesis and secretion of endocrine

but AQP4 might have little contribution to the secretion of pancreatin.

关键词

胰腺水通道蛋白-4免疫组化原位杂交大鼠

Keywords

pancreasaquaporin-4immunohistochemicalIn situ hybridizationrat

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