Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China

doi:10.13406/j.cnki.cyxb.2007.05.029

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Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China

更新时间：2024-01-17

- Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China
- Journal of Chongqing Medical University Issue 5, Pages: 464-466(2007)
- 作者机构：
  
  重庆医科大学附属第一医院耳鼻咽喉科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.05.029
  CLC： R739.63
- Published：2007，
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[1]杨玉成,王祎琴,钱迪,何芸,洪苏玲.重庆地区鼻咽癌患者血浆EB病毒LMP1基因检测及N端XhoI酶切位点变异分析[J].重庆医科大学学报,2007(05):464-466+473.

Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China. [J]. Journal of Chongqing Medical University (5):464-466(2007)
[1]杨玉成,王祎琴,钱迪,何芸,洪苏玲.重庆地区鼻咽癌患者血浆EB病毒LMP1基因检测及N端XhoI酶切位点变异分析[J].重庆医科大学学报,2007(05):464-466+473. DOI： 10.13406/j.cnki.cyxb.2007.05.029.

Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China. [J]. Journal of Chongqing Medical University (5):464-466(2007) DOI： 10.13406/j.cnki.cyxb.2007.05.029.

摘要

目的:研究重庆地区鼻咽癌和非鼻咽癌患者血浆Epstein-Barr病毒（EBV）潜伏膜蛋白l（LMP1）基因存在情况及N端XhoI酶切位点变异状况

探讨其在鼻咽癌发生中所起的作用。方法:收集重庆籍鼻咽癌患者外周血48例

非鼻咽癌患者外周血40例

提取DNA后

用聚合酶链反应技术（PCR）特异性地扩增LMP1基因的N端区

PCR产物经XhoI酶切后用8%聚丙烯酰胺凝胶（PAGE）电泳分离分析

用双脱氧终止法对部分PCR产物进行测序

用DNAssist软件对序列进行碱基缺失变异分析。结果:48例鼻咽癌外周血中

全部扩增出267bp的特异性LMP1基因条带

阳性率为100%。40例非鼻咽癌者外周血中

38例扩增出特异性条带

阳性率为95%。与B95-8原型LMP1比较

全部PCR产物酶切电泳分析、测序及软件序列分析未发现一例存在XhoI酶切位点缺失变异。结论:我国重庆地区鼻咽癌和非鼻咽癌患者血浆携带的EB病毒LMP1基因XhoI酶切位点无缺失变异;LMP1基因N端XhoI酶切位点缺失变异与鼻咽癌发病的确切关系还需进一步研究。

Abstract

Objective:To detect and analyze the Epstein-Barr virus LMPI gene XhoI-site mutation in plasma of patients with nasopharyngeal carcinoma(NPC)in Chongqing of China.Methods:DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China.All the PCR production was digested by enzyme XhoI

then segregated in 8% PAGE.XhoI-site mutation was analyzed by sequencing and comparing with B95-8 cell.Results: The LMP1 was amplified and digested successfully from 48 of 48 NPC cases(100%)and from 38 of 40 non-NPC cases (95%)

but none of LMPI XhoI-site mutation was found.Sequencing was performed on 4 LMPI genes from NPC cases and 5 LMP1 genes from non-NPC cases

they showed the presence of the XhoI-site.Conclusions:XhoI-site in EBV LMP1 gene N terminus has no mutation in plasma of NPC or non-NPC cases in Chongqing of China.The precise relationship of XhoI-site mutation and NPC pathogenesis need further investigation.

关键词

Epstein-Barr病毒潜伏膜蛋白l鼻咽癌基因突变

Keywords

Epstein-Ban virusLatent membrane protein 1nasopharyngeal carcinomagene mutation

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