Objective:To evaluate the treatment effects of rALR combined with newborn rat hepatocyte through intrasplenic trans- plantation in rats with acute hepatic failure induced by chemical drug.Methods:Acute hepatic failure(AHF)animals were in- duced by 1.2g/kg D-gal and randomly divided into 6 groups after intoxication.Group I as model control received no further treatment

Group II received 1ml buffered saline

groupⅢ1ml rALR

group IV 2x10~7/ml hepatocytes

group V 2x10~7hepa- tocytes suspended in lml rALR(50μg/(kg·d))and group VI 2x10~7hepatocytes in 1ml cyclosporine A(10mg/(kg·d))

through intrasplenic injection

respectively.The abdominal cavities of groupⅡ-Ⅵrats were injected with above same reagents every day till rat death or two weeks.We determined the surviving of rats in each group daily.Concurrently

surviving rats from correspondingly treated groups were killed for histology 1、5 days after operation and subsequently at weekly intervals up to 3 week to obtain histologic specimens for observing liver histopathological change.Result:The model control group rats could have survived for 88 hours at most

which proved intoxication successful.GroupⅢrats’death times were similar to group I and there was no significant difference in survival between the two groups rats.GroupⅢrats could have survived for 90 hours at most

and the liver pathological state and hepatic function change were basically the same as groupⅡrats.A proportion of group IV rats had survived for 2 weeks

and the surviving for 2 weeks was 33.3%.At 2 weeks

the survival of group V rats was significantly higher than that of groups I～IV rats(75% vs 33.3%

P<0.02)

meanwhile

it showed no statistically significant in- crease compared to that of group VI rats(75% vs 43.75%

P=0.07>0.05).there was no significant difference in survival between group IV and group VI rats(P>0.05).Hepatic function and liver pathological state for groups IV

V

VI rats had improvment

especially

group V.Conclusion:It was significant that the effect of treatment of newborn rat’s hepatocyte transplantation in combination with rALR on rat with acute hepatic function failure.It could improve the survival of rats with acute hepatic failure (AHF)induced by D-gal

and ameliorate hepatic function and hepatic pathological state.