Study on of metallo-β-1actamases in pseudomonas aeruginosa

doi:10.13406/j.cnki.cyxb.2007.05.007

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Study on of metallo-β-1actamases in pseudomonas aeruginosa

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- Study on of metallo-β-1actamases in pseudomonas aeruginosa
- Journal of Chongqing Medical University Issue 5, Pages: 497-499(2007)
- 作者机构：
  
  1. 重庆医科大学附属第二医院检验科
  2. 江津市094医院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2007.05.007
  CLC： R378.991
- Published：2007，
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[1]吴倩,陈志,夏亭亭.产金属β内酰酶铜绿假单胞菌的表型和基因型分析[J].重庆医科大学学报,2007(05):497-499.

Study on of metallo-β-1actamases in pseudomonas aeruginosa. [J]. Journal of Chongqing Medical University (5):497-499(2007)
[1]吴倩,陈志,夏亭亭.产金属β内酰酶铜绿假单胞菌的表型和基因型分析[J].重庆医科大学学报,2007(05):497-499. DOI： 10.13406/j.cnki.cyxb.2007.05.007.

Study on of metallo-β-1actamases in pseudomonas aeruginosa. [J]. Journal of Chongqing Medical University (5):497-499(2007) DOI： 10.13406/j.cnki.cyxb.2007.05.007.

摘要

目的:了解我院铜绿假单胞菌的耐药与流行病学状况

以便更好控制医院感染。方法:采用纸片法测定产金属B-内酰酶的PA

并用随机扩增多态性（RAPD）DNA基因分型方法对其进行PAPD基因分型。结果:24株耐亚胺培南PA通过纸片法检测确定产金属β-内酰酶19株

不产金属β-内酰酶5株

产金属B-内酰酶检出率80%;PAPD法共得PAPD十型

分型率为100%。有3例感染个体出现相同基因型

有1例病人在不同时期出现两种基因型。结论:纸片法检测能快速简便检测出金属β-内酰酶

随机扩增多态（PAPD）DNA基因分型

能满足对PA进行分子流行病学调查。

Abstract

Objective:To monitor the rug resistance and epidemiology state of pseudomonas aeruginosa(PA)isolated clinical pa- tient.Methods:antimicrobial sensitivity tests were performed by K-B method and metallo-β-lactamases-producing PA was genotyped by the randomly amplified polymorphic(PAPD)assay.Results:Among24 strains resistant to imipenem.19(8%)strains produced metal enzyme which could be distingurished into 10 kinds of PAPD type.the type ability was 100%.There was same gene type strain.Isolated from three patient and there were two kinds of gene types. Among 2 strains isolated from the same patient at the different time.Conclusions:the K-B method could be used to determine metal enzyme

rapidly and eonviently. And PAPD could to be used in the molecular epidemiological study of PA.

关键词

铜绿假单胞菌金属β-内酰胺酶基因型医院感染

Keywords

Pseudomonas aeruginosaMetalloβ-lactamasesgenetypeHospital infection

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