Objective; To establish a method for content determinate of Rutin in H ibiscus mutabilis L by RT- HLPC. Methods; The chromatographic conditions were as follows; Column; Ubondpak C18; the column temperature was 25℃and the detection wavelength was 370nm;Mobil phase consisted of 0.02mol/L KH2PO4 solution cotaining 1.0% glacial acetic acid -methanol (73:27

v:v). The flow rate was 1.0ml/min. SPD -2ASUV detector was used. The standard sample was used as control. Results; Good linearity was obtained for Rutin and other components in the range of (0.5-5) μg/20μl. Peak area was favorable liner between the concentration of Rutin in range . The regression equation was Y =0.0001X +0. 1208

correlation coefficient r = 99.8%

The recovery rate was 99. 88%

RSD was 1. 54% (n = 5 ). Conclusion; The method is convenient

precise

resulting in high separate efficacy

and suited for the content determination of Rutin in H ibiscus mutabilis L.