Experimental Study on Yisheng Injection for Reducing Cold Ischemia Injury in Isolated Rat Heart

Li Ming; et al

doi:10.13406/j.cnki.cyxb.2005.06.014

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Experimental Study on Yisheng Injection for Reducing Cold Ischemia Injury in Isolated Rat Heart

更新时间：2024-01-17

- Experimental Study on Yisheng Injection for Reducing Cold Ischemia Injury in Isolated Rat Heart
- Journal of Chongqing Medical University Issue 6, Pages: 831-835(2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院普外科
  2. 四川大学华西医院普外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.06.014
  CLC： R285.5
- Published：2005，
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[1]李明,严律南.益生注射液抗大鼠离体心脏冷缺血损伤的实验研究[J].重庆医科大学学报,2005(06):831-835.

Li Ming, et al. Experimental Study on Yisheng Injection for Reducing Cold Ischemia Injury in Isolated Rat Heart. [J]. Journal of Chongqing Medical University (6):831-835(2005)
[1]李明,严律南.益生注射液抗大鼠离体心脏冷缺血损伤的实验研究[J].重庆医科大学学报,2005(06):831-835. DOI： 10.13406/j.cnki.cyxb.2005.06.014.

Li Ming, et al. Experimental Study on Yisheng Injection for Reducing Cold Ischemia Injury in Isolated Rat Heart. [J]. Journal of Chongqing Medical University (6):831-835(2005) DOI： 10.13406/j.cnki.cyxb.2005.06.014.

摘要

目的:探讨中药益生注射液对大鼠离体心脏冷缺血损伤的保护作用

为临床应用提供部分实验依据。方法:采用雌性封闭群SD大鼠18只

分两组

对照组的离体心脏用4℃生理盐水保存

实验组的离体心脏则用含益生注射液的4℃生理盐水保存

分别于冷藏2、6、12、24、48和72h取材作酶组织化学检查。结果:对照组

冷藏24h

碱性磷酸酶（ALP）、辅酶Ⅱ黄递酶（NADPHD）活性开始下降;冷藏48h后

乳酸脱氢酶（LDH）活性开始下降

ALP和NADPHD活性明显下降;冷藏72h后

琥珀酸脱氢酶（SDH）和细胞色素氧化酶（CCO）活性开始下降

NADPHD活性几乎完全丧失;实验组

冷藏24h、48h和72h

NAD- PHD、LDH和CCO活性分别开始下降

但不如对照组明显;冷藏48h后

ALP活性才开始下降;冷藏72h

SDH活性无变化。结论:冷缺血可引起离体心肌损伤

其中血管内皮细胞对冷缺血损伤最敏感

益生注射液对离体心肌冷缺血损伤具有保护作用。

Abstract

Objective;To investigate whether the traditional Chinese medicine Yisheng Injection can reduce the injury on isolated rat heart induced by cold ischemia. Methods ;18 isolated female closed colony SD rats were divided into two groups at random. In the control group

the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃

while in the experimental group

the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃. The specimens were harvested 2

48 and 72 hours after preservation. Histochemical changes were observed in two groups. Results ;In the control group

the activities of alkaline phosphatase ( ALP) and NADPH diaphorase (NADPHD) decreased after cold ischemia for 24 hours. Lactic dehydro-genase (LDH) decreased slightly

while ALP and NADPHD decreased evidently

after cold ischemia for 48 hours. Succinic dehydro-genase ( SDH) and cytochrome oxidase ( CCO) decreased slightly

and NADPHD disappeared after cold ischemia for 72 hours respectively. In the experimental group

NADPHD

LDH and CCO decreased slightly after cold ischemia for 24

48 and 72 hours. ALP decreased slightly after cold ischemia for 48 hours

however

SDH showed no changes

after cold ischemia for 72 hours. Conclusion : Cold ischemia can induce cold ischemia injury in isolated rat hearts

especially the vascular endothelial cells being more sensitive. Yisheng injection can reduce this kind of injury to some degree.

关键词

益生注射液冷缺血损伤大鼠酶组织化学

Keywords

Yisheng injectionCold ischemia injuryclosed colony SD ratEnzyme histochemical staining

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Related Institution

Department of Gereral Surgery,the First Affiliated Hospital,Chongqing Medical University

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四川大学基础医学与法医学院免疫学教研室

四川大学华西医院移植工程和移植免疫实验室

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