Chromosome Aberration During Malignant Transformation of Human Bronchial Epithelial Cells Induced by NNK

KONG Lingwen; et al

doi:10.13406/j.cnki.cyxb.2005.06.010

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Chromosome Aberration During Malignant Transformation of Human Bronchial Epithelial Cells Induced by NNK

更新时间：2024-01-17

- Chromosome Aberration During Malignant Transformation of Human Bronchial Epithelial Cells Induced by NNK
- Journal of Chongqing Medical University Issue 6, Pages: 813-819(2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院胸心外科
  2. 重庆医科大学附属第一医院胸心外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.06.010
  CLC： R734.2
- Published：2005，
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[1]孔令文,吴庆琛,朱进国.人支气管上皮细胞恶性转化过程中染色体畸变的研究[J].重庆医科大学学报,2005(06):813-819.

KONG Lingwen, et al. Chromosome Aberration During Malignant Transformation of Human Bronchial Epithelial Cells Induced by NNK. [J]. Journal of Chongqing Medical University (6):813-819(2005)
[1]孔令文,吴庆琛,朱进国.人支气管上皮细胞恶性转化过程中染色体畸变的研究[J].重庆医科大学学报,2005(06):813-819. DOI： 10.13406/j.cnki.cyxb.2005.06.010.

KONG Lingwen, et al. Chromosome Aberration During Malignant Transformation of Human Bronchial Epithelial Cells Induced by NNK. [J]. Journal of Chongqing Medical University (6):813-819(2005) DOI： 10.13406/j.cnki.cyxb.2005.06.010.

摘要

目的:研究在烟草特异性亚硝胺（NNK）诱发人支气管上皮细胞（BEAS-2B）恶性转化过程中染色体畸变

探讨其在吸烟高危人群罹患肺癌预警及普查方面的意义。方法用NNK诱发BEAS-2B细胞（BEAS-2BNNK）恶性转化

并在此过程中用常规中期染色体分析方法

动态观察染色体畸变的情况。结果:1．NNK诱发BEAS-2B细胞恶性转化模型的建立①BEAS -2BNNK（实验组）第5代细胞血清抗性显著增强。②第15代BEAS-2BNNK细胞具有锚着独立性生长特性。③第20代BEAS -2BNNK细胞超微结构出现明显异型性。④第25代BEAS-2BNNK细胞在裸鼠体内成瘤

病理为高分化鳞形细胞癌。2．染色体畸变①BEAS-2B（对照组）二倍体细胞占细胞总数的91％-97％

传代后核型稳定;从第5代开始BEAS-2BNNK细胞即逐渐失去正常核型

随着传代次数增加

二倍体细胞比例从83％递减至54％

异倍体及多倍体细胞呈递增趋势

较对照组明显增高。②各代BEAS-2B细胞结构畸变发生率为2％-4％;除第5代BEAS-2BNNK细胞结构畸变总频率（11％）明显高于BEAS -2B细胞外

其余各代细胞与BEAS-2B细胞相近。结论:NNK能成功诱发人支气管上皮细胞（BEAS-2B）细胞恶性转化

为进一步探讨肺癌发生机制、尤其是吸烟致肺癌发生机制提供了理想模型。染色体的异倍性和多倍性在NNK诱发BEAS- 2B细胞转化成肺癌过程中可能属早期事件

有望成为吸烟高危人群罹患肺癌的预警及普查指标之一。

Abstract

Objective To investigate chromosome aberration during malignant transformation of immortalized human bronchial epithelial cells ( BEAS - 2B) induced by tobacco specific nitrosamine ( NNK)

and explore those significance of early - warning and mass screening of lung cancer in high - risk smoking population. Methods Immortalized human bronchial epithelial cells ( BEAS - 2B) were induced to malignant transformation cells ( BEAS - 2BNNK ) by NNK

and chromosome aberration were detected in the different passage of BEAS - 2BNNK and BEAS - 2B cells by analysis of metaphase chromosome. Results 1. Model of malignant transformation of BEAS -2B cells induced by NNK was created (1)The serum resistance was significantly increased in the 5th passage BEAS - 2BNNK cells.(2) The anchorage independent growth was appeared in the 15th passage. (3)The ultrastructure of the 20th passage BEAS - 2BNNK cells shown obvious heteromorphy characterization. (4)The 25th passage BEAS -2BNNK cells formed tumors in nude mice

and tumors were overdifferentiation squamous cell carcinoma confirmed by histopathological examination. 2. Chromosome aberration (1) BEAS -2B cells hold relatively stable diploid karyotype which ratio was 91%-97% up to the 25th passage. Different passage BEAS -2BNNK cells gradually lost the normal diploid karyotype and the ratio of diploid cells decreased from 83% to 54%

the proportion of polyploid and aneu-ploid cells increased in process of passing generation. (2)The ratio of structure aberration neared to 2% ~ 4% in BEAS - 2BNNK and BE-AS -2B cells but the 25th passage of BEAS -2BNNK cells was 11%. Conclusion The model of malignant transformation of BEAS - 2B cells induced by NNK could be created successfully and provided for investigating the molecular biological mechanism of lung cancer

especially smoking - related cases. The polyploid and aneuploid chromosomes could be the early event in the process of malignant transformation of BEAS - 2B cells induced by NNK. In a word

the chromosome aberration might be useful markers for the early warning and mass screening of lung cancer

especially in high - risk smoking population.

关键词

NNK永生化人支气管上皮细胞（BEAS-2B）染色体畸变

Keywords

NNKImmortalized human bronchial epithelial cells (BEAS -2B)ChromosomeAberration

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