Cellular localization of SARS - CoV nucleocapsid protein in 293 cells

TAO Peng et al

doi:10.13406/j.cnki.cyxb.2005.06.009

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Cellular localization of SARS - CoV nucleocapsid protein in 293 cells

更新时间：2024-01-17

- Cellular localization of SARS - CoV nucleocapsid protein in 293 cells
- Journal of Chongqing Medical University Issue 6, Pages: 808-812(2005)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所感染性疾病分子生物学教育部重点实验室
  2. 重庆医科大学病毒性肝炎研究所感染性疾病分子生物学教育部重点实验室,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.06.009
  CLC： R373
- Published：2005，
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[1]陶鹏,陈维贤,郭进军,黄爱龙.SARS冠状病毒N蛋白的真核细胞定位研究[J].重庆医科大学学报,2005(06):808-812.

TAO Peng et al. Cellular localization of SARS - CoV nucleocapsid protein in 293 cells. [J]. Journal of Chongqing Medical University (6):808-812(2005)
[1]陶鹏,陈维贤,郭进军,黄爱龙.SARS冠状病毒N蛋白的真核细胞定位研究[J].重庆医科大学学报,2005(06):808-812. DOI： 10.13406/j.cnki.cyxb.2005.06.009.

TAO Peng et al. Cellular localization of SARS - CoV nucleocapsid protein in 293 cells. [J]. Journal of Chongqing Medical University (6):808-812(2005) DOI： 10.13406/j.cnki.cyxb.2005.06.009.

摘要

目的:为进一步探讨SARS-CoV核衣壳蛋白（N蛋白）在病毒复制及细胞通路中的作用

观察了核衣壳蛋白不同区段在细胞内的定位。方法:应用核定位序列分析软件分析N蛋白序列

找出其中的核定位信号序列;构建增强型绿色荧光蛋白（EGFP）与SARS-CoV不同长度核衣壳蛋白区段融合表达的pEGFP-C1重组载体

对293细胞进行瞬时转染

在荧光显微镜下观察核衣壳蛋白不同区段在细胞内的定位。结果:不同的核定位信号分析软件分析的结果不同

Prosite找到的核定位信号位于N蛋白第373-389或374-390aa

而PredietNLS server找到的核定位信号位于N蛋白第36-44aa;全长核衣壳蛋白及第161-422aa区段均定位于细胞质

第1-187aa区段定位于细胞质和细胞核。结论:SARS-CoV核衣壳蛋白的核定位信号可能位于N蛋白第36-44aa。

Abstract

Objective; To study the role of nucleocapsid protein in virus replication and cell signaling passway

and to observe the cellular localization of SARS - CoV nucleocapsid protein. Methods; The sequence of SARS - CoV nucleocapsid protein was analyzed by protein analysis programs to identify nuclear localization signals ( NLS); 293 cells were transfected by the pECFP - C1 recombinant plasmids which expressed fusion protein of enhanced green fluorescence protein(EGFP) and nucleocapsid protein. At 24

72 hours post transfection (p. t. )

the Green Fluorescent Proteins (GFP) were observed by fluorescence microscope. Results; By using PROS-ITE

we identified a nuclear localization signal sequence from amino acids 373 to 389 or from amino acids 374 to 390 in the nucleocapsid protein sequence. However

the analysis of PredictNLS server revealed that the nuclear localization signal region was located between amino acids 36 and 44. N1 protein( 1 - 187aa) was located in both cytoplasm and cell nucleu;N protein(1 -422aa) and N2 protein( 161 -422aa) were located in cytoplasms . Conclusion; The nuclear localization signal is likely to be located between amino acids 36 and 44 in SARS - CoV nucleocapsid protein.

关键词

SARS-CoV核衣壳蛋白细胞定位

Keywords

SARS-CoVNucleocapsid proteinLocalization

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