Resistance of different methods of hemoglobin assay to common interference

CHENG Chaoying; et al

doi:10.13406/j.cnki.cyxb.2005.04.030

您当前的位置：

首页 >

文章列表页 >

Resistance of different methods of hemoglobin assay to common interference

更新时间：2024-01-17

- Resistance of different methods of hemoglobin assay to common interference
- Journal of Chongqing Medical University Issue 4, Pages: 591-593(2005)
- 作者机构：
  
  1. 重庆市第八人民医院内科
  2. 重庆医科大学附属第一医院
  3. 重庆医科大学生物化学教研室
  5. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.030
  CLC： R446.1
- Published：2005，
扫描看全文
[1]程朝英,廖蓉,夏丽,李生兵,郭睿,赵利娜,廖飞.血红蛋白测定方法抗干扰能力的比较[J].重庆医科大学学报,2005(04):591-593.

CHENG Chaoying, et al. Resistance of different methods of hemoglobin assay to common interference. [J]. Journal of Chongqing Medical University (4):591-593(2005)
[1]程朝英,廖蓉,夏丽,李生兵,郭睿,赵利娜,廖飞.血红蛋白测定方法抗干扰能力的比较[J].重庆医科大学学报,2005(04):591-593. DOI： 10.13406/j.cnki.cyxb.2005.04.030.

CHENG Chaoying, et al. Resistance of different methods of hemoglobin assay to common interference. [J]. Journal of Chongqing Medical University (4):591-593(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.030.

摘要

目的:探讨抗氧化体系干预物质和维生素C（VC）对血红蛋白（Hb）测定的干扰。方法:兔红细胞溶血后为样品。比较抗氧化酶抑制剂和VC对直接测定Hb、测定碱化Hb、测定叠氮Hb等的干扰。结果:直接测定Hb吸收、测定叠氮Hb都受到VC、超氧化物歧化酶抑制剂、过氧化氢酶抑制剂等的干扰

碘乙酰胺和谷胱甘肽过氧化物酶抑制剂干扰较小。上述物质对测定碱化Hb几乎没有干扰。结论:测定碱化Hb用于评价高浓度VC的溶血作用和机制更可靠。

Abstract

Objective:To investigate the effects of common inhibitors of the antioxidation system and vitamin C on the measurement of hemoglobin (Hb).Methods:Hemolyzed rabbit red blood cell was used as the sample.The interferences of common inhibitors of the antioxidation system on assay of Hb by its absorbance at 540nm

alkalinized Hb by absorbance at 575nm

the azide-Hb complex by absorbance at 542nm were compared.Results:Vitamin C and the inhibitors of superoxide dismutase

catalase exhibited larger interferences with direct assay of Hb by absorbance at 540 nm and the assay of azide-Hb

and inhibitor of GSH and GSH peroxidase showed smaller effects.The assay of alkalinized Hb exhibited the strongest resistance to all these chemicals.Conclusion:The assay of alkalinized Hb shows the strongest resistance to common chemicals used to investigate the mechanism of peroxidative damage and can be applicable to the study of the mechanism of vitamin C-induced hemolysis.

关键词

血红蛋白测定维生素C抗氧化体系碱化血红蛋白溶血

Keywords

Hemoglobin assayvitamin CAntioxidation systemAlkalinized hemoglobinHemolysis

references

Views

235

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The effects of Vitamin C on urinary albumin excretion rate in rats with streptozotocin-induced diabetes

The effects of high dosage vitamin C on myocardial mitochondrial structure and function in mice with viral myocarditis

Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection

Study on the best time of giving vitamin C after 131I therapy in patients with differentiated thyroid cancer

Related Author

肖谦

汪恕萍

何军

CHEN Jianbin

et al

常静

廖晓岗

ZHU Xiaoyun

Related Institution

重庆医科大学临床学院内分泌科

Department of Internal Medicine, College of Clinical Medicine, Chongqing Medical University

重庆医科大学临床学院内科

Department of Biochemistry,College of Basic Medicine,Chongqing Medical University

重庆医科大学2001级

⁰