Study on the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat’s liver cells

GONG Ming; et al

doi:10.13406/j.cnki.cyxb.2005.04.023

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Study on the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat’s liver cells

更新时间：2024-01-17

- Study on the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat’s liver cells
- Journal of Chongqing Medical University Issue 4, Pages: 567-569(2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院老年病科内分泌组
  2. 重庆医科大学附属第一医院老年病科内分泌组,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.023
  CLC： R341
- Published：2005，
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[1]龚明,李超林,肖谦,黄昶荃.脂联素对大鼠肝细胞葡萄糖激酶与磷酸烯醇式丙酮酸羧激酶活性影响的研究[J].重庆医科大学学报,2005(04):567-569.

GONG Ming, et al. Study on the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat’s liver cells. [J]. Journal of Chongqing Medical University (4):567-569(2005)
[1]龚明,李超林,肖谦,黄昶荃.脂联素对大鼠肝细胞葡萄糖激酶与磷酸烯醇式丙酮酸羧激酶活性影响的研究[J].重庆医科大学学报,2005(04):567-569. DOI： 10.13406/j.cnki.cyxb.2005.04.023.

GONG Ming, et al. Study on the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat’s liver cells. [J]. Journal of Chongqing Medical University (4):567-569(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.023.

摘要

目的:探讨脂联素对体外培养大鼠肝脏细胞葡萄糖激酶与磷酸烯醇式丙酮酸羧激酶活性影响

及其在胰岛素抵抗中的意义。方法:以BRL肝脏细胞株为细胞模型

分别用10μg/ml、30μg/ml、50μg/ml脂联素处理细胞

用6-磷酸葡萄糖脱氢酶偶联比色法、乳酸脱氢酶偶联比色法检测大鼠肝脏细胞葡萄糖激酶与磷酸烯醇式丙酮酸羧激酶活性。结果:与对照组比较

磷酸烯醇式丙酮酸羧激酶在脂联素处理24h后活性明显降低

且随脂联素浓度升高作用越明显

P<0.05。葡萄糖激酶在脂联素处理24小时后

活性与对照组比较没有显著性差异

P>0.5。结论:脂联素对葡萄糖激酶无明显的调节作用

而对于磷酸烯醇式丙酮酸羧激酶具有显著的抑制作用。脂联素通过对肝细胞磷酸烯醇式丙酮酸羧激酶活性的调节

降低肝脏葡萄糖异生

减少肝糖输出

改善胰岛素抵抗。

Abstract

Objective:To investigate the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat’s liver cells.Methods:The BRL was used as cell model.The rat liver cells were incubated with adiponectin of three different concentrations (10μg/ml

30μg/ml and 50μg/ml).Controlled cells were incubated without adiponectin.Enzymatic activity of GK was investigated by using a standard G6P-coupled assay.Enzymatical activity of PEPCK was assessed by using a standard LDH-coupled assay.Results:Compared with the control group

treated group with adiponectin of all concentrations for 24h had no significant effect on enzymatic activity of glucokinase.Enzymatic activity of phosphoenolpyruvate carboxykinase was significantly decreased even in the lowest concentration of adiponectin

and along with increased in adiponectin concentration enzymatic activity of phosphoenolpyruvate carboxykinase decreased more significantly.Conclusion:Adiponectin has no significant effect on ezymatic activity of glucokinase.However adiponectin decreases enzymatic activity of phosphoenolpyruvate carboxykinase significantly.Through this effect

adiponectin decreases liver glucose production and improves insulin resistance.

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脂联素葡萄糖激酶磷酸烯醇式丙酮酸羧激酶胰岛素抵抗

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