The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB-231 in vitro

LIU Fangxin; et al

doi:10.13406/j.cnki.cyxb.2005.04.022

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The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB-231 in vitro

更新时间：2024-01-17

- The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB-231 in vitro
- Journal of Chongqing Medical University Issue 4, Pages: 564-566(2005)
- 作者机构：
  
  1. 重庆医科大学基础医学院核医学教研室
  2. 重庆医科大学基础医学院核医学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.022
  CLC： R737.9
- Published：2005，
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[1]刘方欣,张玉诺,李少林.环氧化酶抑制剂对人乳腺癌细胞株MCF-7的抑制作用研究[J].重庆医科大学学报,2005(04):564-566+580.

LIU Fangxin, et al. The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB-231 in vitro. [J]. Journal of Chongqing Medical University (4):564-566(2005)
[1]刘方欣,张玉诺,李少林.环氧化酶抑制剂对人乳腺癌细胞株MCF-7的抑制作用研究[J].重庆医科大学学报,2005(04):564-566+580. DOI： 10.13406/j.cnki.cyxb.2005.04.022.

LIU Fangxin, et al. The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB-231 in vitro. [J]. Journal of Chongqing Medical University (4):564-566(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.022.

摘要

目的:研究环氧化酶抑制剂阿司匹林和选择性环氧化酶2抑制剂尼美舒利对人乳腺癌细胞株MDA-MB-231的生长抑制作用

并初步探讨其作用机理。方法:用阿司匹林和尼美舒利分别作用人乳腺癌细胞株MDA-MB-231

MTT法观察其生长抑制作用;免疫组化法检测阿司匹林和尼美舒利作用后MDA-MB-231细胞中COX-2和c-erbB-2的表达情况。结果:阿司匹林和尼美舒利能够抑制MDA-MB-231细胞的生长

并呈时间-剂量依赖型。COX-2在MDA-MB-231细胞中表达呈阳性

免疫组化发现细胞内c-erbB-2表达下降。结论:环氧化酶抑制剂能够有效的抑制肿瘤细胞的生长

其机制可能与下调c-erbB-2基因的表达有关。

Abstract

Objective:To investigate the inhibitory effect of cyclooxygenase inhibitor acetylsalicylic acid(ASA)

and a selective COX-2 inhibitor(Nimesulide) on the growth of human breast cancer cell line MDA-MB-231 in vitro.Methods:The inhibitory effect was detected by MTT assay.Immunohistochemical assay was performed to examine the expressions of COX-2 and c-erbB-2 in SKOV3 cell line treated by NSAIDs.Results:A decrease in cell number compared with controls was observed in all of the cell line treated with ASA and Nimesulide.It was a dose-dependent inhibition of cell proliferation.COX-2 was expressed positive in MDA-MB-231 and the expression of cerbB-2 was found to decrease by immunohistochemical assay.Conclusion:NSAIDs can inhibit the growth of human breast cancer cell line MDA-MB-231 in vitro.

关键词

环氧化酶抑制剂选择性环氧化酶2抑制剂人乳腺癌细胞株MDA-MB-231

Keywords

Cyclooxygenase inhibitor acetylsalicylic acidSelective COX-2 inhibitorHuman breast cancer cell line MDA-MB-231

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