Growth of mycobacterium tuberculosis H37Ra in the organs of mice after immunization

XIA Changsheng; et al

doi:10.13406/j.cnki.cyxb.2005.04.021

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Growth of mycobacterium tuberculosis H37Ra in the organs of mice after immunization

更新时间：2024-01-17

- Growth of mycobacterium tuberculosis H37Ra in the organs of mice after immunization
- Journal of Chongqing Medical University Issue 4, Pages: 561-563(2005)
- 作者机构：
  
  1. 重庆医科大学检验系临床免疫学教研室
  2. 重庆医科大学检验系临床免疫学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.021
  CLC： R392
- Published：2005，
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[1]夏长胜,卢贤瑜,陈思静.结核分枝杆菌H37Ra免疫小鼠后细菌在体内定植的研究[J].重庆医科大学学报,2005(04):561-563.

XIA Changsheng, et al. Growth of mycobacterium tuberculosis H37Ra in the organs of mice after immunization. [J]. Journal of Chongqing Medical University (4):561-563(2005)
[1]夏长胜,卢贤瑜,陈思静.结核分枝杆菌H37Ra免疫小鼠后细菌在体内定植的研究[J].重庆医科大学学报,2005(04):561-563. DOI： 10.13406/j.cnki.cyxb.2005.04.021.

XIA Changsheng, et al. Growth of mycobacterium tuberculosis H37Ra in the organs of mice after immunization. [J]. Journal of Chongqing Medical University (4):561-563(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.021.

摘要

目的:了解H37Ra（人型结核分枝杆菌标准无毒株）免疫小鼠后在体内生长情况。方法:将H37Ra和BCG分别皮内接种C57BL/6小鼠

免疫后15天、30天及60天

取稀释小鼠脾脏和肺脏匀浆接种罗氏培养基

培养18天后计菌落数（CFU）。结果:免疫后15天、30天及60天

H37Ra在小鼠脾脏、肺脏定植的平均Log10CFU为1.95、1.57、2.54和1.37、1.08及0.94;BCG在小鼠脾脏、肺脏定植的平均Log10CFU则为1.72、1.39、1.06和1.16、0.96及0.50。免疫后15天及30天

脾脏及肺脏定植的H37Ra和BCG无显著性差异（P>0.05）;免疫后60天

脾脏及肺脏定植的H37Ra显著多于BCG（P<0.05）。H37Ra和BCG在脾脏定植的细菌数显著多于肺脏（P<0.05）。结论:H37Ra在C57BL/6小鼠体内至少可以存活2个月。

Abstract

Objective:To investigate the growth of H37Ra in the spleen and lung of mice.Methods:C57BL/6 mice were subcutaneously immunized with H37Ra and BCG respectively. At various intervals （15 days

30 days

60 days） after immunization

the spleen and lung of mice were isolated

dissociated and suitable dilutions of the homogenates were plated on Lowenstein-Jensen medium.The numbers of CFU were counted 18 days after incubation.Results:H37Ra (mean Log

CFU) locating in the spleen were 1.95

1.57 and 2.54;those growing in the lung were 1.37

1.08 and 0.94.The number of BCG locating in the spleen were 1.72

1.39 and 1.06;those growing in the lung were 1.16

0.96 and 0.50.At fifteenth and thirtieth days after immunization

the number of H37Ra and BCG locating in the spleen or lung did not show any significant difference （P

0.05）.But at sixtieth day after immunization

the number of H37Ra growing in the spleen or lung was significantly larger than those of BCG（P

0.05）.The number of H37Ra or BCG locating in the spleen were significantly larger than those growing in the lung（P

0.05）.Conclusion:H37Ra can exist at least for two months in the spleen and lung of C57BL/6 mice.

关键词

H37RaBCG结核疫苗

Keywords

H37RaBCGtuberculosisvaccine

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