The comparison between the action of <sup>131</sup>I-GMCSF and <sup>131</sup>I-anti-CD45 on HL60

LOU Shifeng; et al

doi:10.13406/j.cnki.cyxb.2005.04.015

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The comparison between the action of ¹³¹I-GMCSF and ¹³¹I-anti-CD45 on HL60

更新时间：2024-01-17

- The comparison between the action of ¹³¹I-GMCSF and ¹³¹I-anti-CD45 on HL60
- Journal of Chongqing Medical University Issue 4, Pages: 536-539(2005)
- 作者机构：
  
  1. 重庆医科大学附属第二医院血液科
  2. 重庆医科大学附属第二医院血液科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.015
  CLC： R733.71
- Published：2005，
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[1]娄世锋,张萍,周慷.~(131)I-GM-CSF与~(131)I-anti-CD45分别作用于HL60细胞的比较[J].重庆医科大学学报,2005(04):536-539.

LOU Shifeng, et al. The comparison between the action of ¹³¹I-GMCSF and ¹³¹I-anti-CD45 on HL60. [J]. Journal of Chongqing Medical University (4):536-539(2005)
[1]娄世锋,张萍,周慷.~(131)I-GM-CSF与~(131)I-anti-CD45分别作用于HL60细胞的比较[J].重庆医科大学学报,2005(04):536-539. DOI： 10.13406/j.cnki.cyxb.2005.04.015.

LOU Shifeng, et al. The comparison between the action of ¹³¹I-GMCSF and ¹³¹I-anti-CD45 on HL60. [J]. Journal of Chongqing Medical University (4):536-539(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.015.

摘要

目的:为了比较相同放射性浓度下的131I-GMCSF和131I-anti-CD45对HL60细胞的细胞毒作用

诱导凋亡作用及对细胞周期的影响。我们采用体外放射免疫导向治疗（RIT）模型

用MTT法比较131I-GMCSF和131I-anti-CD45对HL60细胞的细胞毒作用

Annexin V/PI双染法测两处理组的早期凋亡细胞

并用流式细胞术检测其对细胞周期的影响。结果显示131I-GMCSF和131I-anti-CD45对HL60细胞的细胞毒作用非常接近

当放射性浓度≥18.5×108Bq/L时两处理组的HL60细胞存活率都显著下降

两组都能明显诱导HL60细胞凋亡

能显著引起HL60细胞周期发生G2/M期阻滞

两者相比没有显著性差异（P>0.05）。结论:用GM-CSF作为载体代替通常用的鼠型单克隆抗体用于急性髓性白血病（AML）的放射免疫导向治疗是可行的

具有重要的临床意义。

Abstract

Objective:To compare the cytotoxicity

apoptosis and cell cycle changes of HL60 cells treated with

131

I-GMCSF and

131

I-anti-CD45.Methods:An in vitro radioimmunotherapy （RIT） model was employed.Data were collected from tetrazolium microculture cellar cytotoxicity test （MTT） and flow cytometric analysis.Results:The results showed that

131

I-GMCSF and

131

I-anti-CD45 both induced apoptosis on HL60 cells

and the cells growth inhibition rates were close between the two groups.G2/M arrest was observed in two groups.There was no notable difference between

131

I-GM-CSF group and

131

I-anti-CD45 group（P

0.05）.Conclusion:It is feasible to treat AML by using GM-CSF instead of mouse monoclonal antibody combined

131

关键词

放射免疫导向治疗细胞凋亡GM-CSF

Keywords

Targeted radioimmunotherapyapoptosisGM-CSF

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⁰

The comparison between the action of 131I-GMCSF and 131I-anti-CD45 on HL60

DOI：10.13406/j.cnki.cyxb.2005.04.015

摘要

Abstract

关键词

Keywords

references

The comparison between the action of ¹³¹I-GMCSF and ¹³¹I-anti-CD45 on HL60