Effect of polycystic ovary-like environment on E<sub>2</sub> and P levels in human granulosa cells in vitro

QIU Yan; et al

doi:10.13406/j.cnki.cyxb.2005.04.009

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Effect of polycystic ovary-like environment on E₂ and P levels in human granulosa cells in vitro

更新时间：2024-01-17

- Effect of polycystic ovary-like environment on E₂ and P levels in human granulosa cells in vitro
- Journal of Chongqing Medical University Issue 4, Pages: 516-518(2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院辅助生殖中心
  2. 重庆医科大学附属第一医院辅助生殖中心,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.009
  CLC： R711.7
- Published：2005，
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[1]丘彦,刘敬,周永芳,幸贵邦,王炼炼,桂文武,刘淑文.类PCOS激素环境对颗粒细胞E_2、P分泌功能的影响[J].重庆医科大学学报,2005(04):516-518.

QIU Yan, et al. Effect of polycystic ovary-like environment on E₂ and P levels in human granulosa cells in vitro. [J]. Journal of Chongqing Medical University (4):516-518(2005)
[1]丘彦,刘敬,周永芳,幸贵邦,王炼炼,桂文武,刘淑文.类PCOS激素环境对颗粒细胞E_2、P分泌功能的影响[J].重庆医科大学学报,2005(04):516-518. DOI： 10.13406/j.cnki.cyxb.2005.04.009.

QIU Yan, et al. Effect of polycystic ovary-like environment on E₂ and P levels in human granulosa cells in vitro. [J]. Journal of Chongqing Medical University (4):516-518(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.009.

摘要

目的:研究类PCOS的激素环境对正常颗粒细胞分泌功能的影响。方法:体外培养颗粒细胞按不同激素环境雄烯二酮（A）组、黄体生成素（LH）组、胰岛素（INS）组、A+LH+INS组分为四组

每组分设高浓度（类PCOS环境）和低浓度（类生理环境）组。检测不同激素环境下各组培养液中颗粒细胞E2、P水平的变化。结果:组内比较

类PCOS环境中A组及A+LH+INS组E2、P水平较低（P<0.01）

LH组及INS组E2、P水平较高（P<0.01）。组间比较显示

两种浓度的激素环境中

E2、P水平在LH组I、NS组及A+LH+INS组均明显高于A组

且E2水平在INS组最高而P水平在LH组最高（P<0.01）

类PCOS环境下

A+LH+INS组的E2水平较INS组明显低而P水平较LH组明显降低（P<0.01）;生理浓度环境下

A+LH+INS组E2、P水平与INS组、LH组相比无明显差异。结论:体外类PCOS浓度下

A有抑制正常颗粒细胞合成及分泌E2、P分泌的作用

并可能抑制LHI、NS对颗粒细胞合成E2、P;生理浓度下

A对LH及INS刺激颗粒细胞分泌E2、P的无明显抑制作用。

Abstract

Objective:To study the effect of polycystic ovary-like environment on secretion of E

and P from human granulosa luteal cells in vitro.Methods:According to different additives granulosa cells cultured in vitro which were retrieved from infertility women with normal ovulation who were undergoing in vitro fertilization-embryo transfer（IVF-ET）

were divided into four groups:A （androstenedione） group

LH group

INS （insulin） group and A+LH+INS group.Each group was subdivided into polycystic ovary-like environment group with higher levels of the additives and physiological environment group with lower levels of the additives.Levels of E

and P in medium were detected separately.Results:Levels of E

and P in polycystic ovary-like environment were significantly decreased in A group and A+LH+INS group

and significantly increased in LH group and INS group （P

0.01 respectively）.Levels of E

and P of LH group

INS group and A+LH+INS group were all significantly higher than that of A group

with the highest level of E

in INS group and the highest level of P in LH group.Moreover

compared with INS group or LH group respectively

concentrations of E

and P in A+LH+INS group were both significantly decreased in polycystic ovary-like environment but no difference was found in physiological environment.Conclusion:In polycystic ovary-like environment

and P secretion from normal granulosa luteal cells may be inhibited by A.

关键词

类PCOS激素环境颗粒细胞雌二醇孕激素

Keywords

Polycystic ovary-like environmentGranulosa cellsestradiolProgestrone

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Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University

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重庆医科大学附属第一医院妇产科辅助生殖中心

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Effect of polycystic ovary-like environment on E2 and P levels in human granulosa cells in vitro

DOI：10.13406/j.cnki.cyxb.2005.04.009

摘要

Abstract

关键词

Keywords

references

Effect of polycystic ovary-like environment on E₂ and P levels in human granulosa cells in vitro