Suicide gene therapy for mouse bladder cancer using HSV-tk/GCV system

ZHANG Ronggui; et al

doi:10.13406/j.cnki.cyxb.2005.04.008

您当前的位置：

首页 >

文章列表页 >

Suicide gene therapy for mouse bladder cancer using HSV-tk/GCV system

更新时间：2024-01-17

- Suicide gene therapy for mouse bladder cancer using HSV-tk/GCV system
- Journal of Chongqing Medical University Issue 4, Pages: 513-515(2005)
- 作者机构：
  
  1. 第三军医大学新桥医院泌尿外科
  2. 第三军医大学新桥医院泌尿外科,重庆,400037
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.008
  CLC： R737.14
- Published：2005，
扫描看全文
[1]张荣贵,叶钢,刘为池.HSV-tk/GCV系统对鼠膀胱癌的自杀基因治疗研究[J].重庆医科大学学报,2005(04):513-515.

ZHANG Ronggui, et al. Suicide gene therapy for mouse bladder cancer using HSV-tk/GCV system. [J]. Journal of Chongqing Medical University (4):513-515(2005)
[1]张荣贵,叶钢,刘为池.HSV-tk/GCV系统对鼠膀胱癌的自杀基因治疗研究[J].重庆医科大学学报,2005(04):513-515. DOI： 10.13406/j.cnki.cyxb.2005.04.008.

ZHANG Ronggui, et al. Suicide gene therapy for mouse bladder cancer using HSV-tk/GCV system. [J]. Journal of Chongqing Medical University (4):513-515(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.008.

摘要

目的:在体外和体内水平建立膀胱癌的单纯疱疹病毒-胸苷激酶/丙氧鸟苷（herpes simplex virus type thymidine Ki-nase/ganciclovir

HSV-tk/GCV）自杀基因系统

并验证该系统的有效性。方法:用逆转录病毒载体HSV-TK转染鼠膀胱癌细胞株T739

体外检测其对GCV的敏感性

在同基因鼠

分别建立T739和T739-TK膀胱癌腹腔肿瘤模型。当B超显示肿瘤直径约0.5

.8cm时

腹腔GCV给药6天

然后观察肿瘤大小变化及动物存活时间。结果:GCV对T739-TK细胞有显著杀伤作用

而对T739细胞的生长无影响

成功的建立了T739同基因鼠膀胱癌腹腔肿瘤模型

体内实验得到相应结果

转染了TK基因的T739鼠肿瘤明显变小

甚至消失

而未转染TK基因的T739鼠肿瘤继续增大

GCV对其无明显治疗作用

转染了TK基因的T739鼠存活时间显著延长。结论:在体外和体内水平

表达TK基因的肿瘤细胞均被GCV有效杀伤

这表明HSV-tk/GCV系统有可能成为基因治疗膀胱癌的有效方法。

Abstract

Objective:To establish a suicide gene therapy system HSV-tk/GCV for bladder cancer

a treatment in vitro as well as in vivo and to test its efficacy.Methods:Mouse bladder cancer cell line (T739) was transfected with retroviral vector HSV-tk gene.The sensitivity of T739-TK cells to GCV was detected in vitro.In the mouse model of bladder tumor

T739 or T739-TK was implanted beneath the peritoneum of syngeneic mice.When tumors grew to the size of 0.5～0.8cm

intraperitoneal administration of GCV was carried out for 6 days.Changes of tumor size and survival rate of mice were observed.Results:RT-PCR showed that TK gene was transferred into the T739 cell and expressed successfully. In vitro

T739-TK cells became sensitive to low concentrations of GCV.In vivo studies showed similar result.Significant tumor inhibition was found in the T739-TK group after administration of GCV

and the survival time of mice was prolonged.Conclusion:Tumor cells expressing HSV-tk gene were eradicated by administration of GCV in vitro as well as in vivo.

关键词

HSV-TK基因膀胱癌基因治疗丙氧鸟苷

Keywords

HSV-TKbladder cancergene therapyGanciclovir(GCV)

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of retroviral HSVtk gene transfer on mice bladder tumor growth in vivo

Value of inflammatory，pyroptosis，and ferroptosis biomarkers in the treatment and prognosis of bladder cancer

Correlation between serum KLF5 content and positive lymph node ratio and its predictive value for prognosis of patients with bladder cancer after radical operation

Value of inflammatory biomarkers in predicting the outcome of patients with muscle-invasive bladder cancer receiving different neoadjuvant treatment regimens

Application of the stoma incision-assisted technique in laparoscopic radical cystectomy and ileal conduit

Related Author

YE Gang

et al

张荣贵

Pei Xiaming

Li Hong

Liang Yu

Xie Yu

Chang Haiqing

Related Institution

Department of Urology,Xinqiao Hospital,Third Military Medical University

Department of Urology,Pingxiang People’s Hospital

Department of Urology,Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University

Department of Urology,The Second Hospital,University of South China

Department of Urinary Surgery，Xinxiang Central Hospital，The Fourth Clinical College of Xinxiang Medical University

⁰