Experimental study of VEGF gene transfer combined with deproteinized bone to repair avascular necrosis of the femoral head

CAO Kai; et al

doi:10.13406/j.cnki.cyxb.2005.04.006

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Experimental study of VEGF gene transfer combined with deproteinized bone to repair avascular necrosis of the femoral head

更新时间：2024-01-17

- Experimental study of VEGF gene transfer combined with deproteinized bone to repair avascular necrosis of the femoral head
- Journal of Chongqing Medical University Issue 4, Pages: 505-508(2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院骨科
  2. 重庆医科大学附属儿童医院肾脏免疫科,重庆,400016
  3. ,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.006
  CLC： R681.8
- Published：2005，
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[1]曹凯,黄伟,安洪,蒋电明,黄路.VEGF基因转染结合脱蛋白骨修复股骨头坏死的实验研究[J].重庆医科大学学报,2005(04):505-508+547.

CAO Kai, et al. Experimental study of VEGF gene transfer combined with deproteinized bone to repair avascular necrosis of the femoral head. [J]. Journal of Chongqing Medical University (4):505-508(2005)
[1]曹凯,黄伟,安洪,蒋电明,黄路.VEGF基因转染结合脱蛋白骨修复股骨头坏死的实验研究[J].重庆医科大学学报,2005(04):505-508+547. DOI： 10.13406/j.cnki.cyxb.2005.04.006.

CAO Kai, et al. Experimental study of VEGF gene transfer combined with deproteinized bone to repair avascular necrosis of the femoral head. [J]. Journal of Chongqing Medical University (4):505-508(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.006.

摘要

目的:构建pcDNA3.1/VEGF165质粒及联合脱蛋白骨基因治疗早期股骨头缺血性坏死。方法:将含人VEGF165全长cDNA序列的克隆载体pUC18/VEGF165与pcDNA3.1（+）载体构建成重组真核表达质粒pcDNA3.1/VEGF165;69只AVNFH造模成功后的新西兰大白兔随机分成3组。第一组

将脱蛋白骨复合pcDNA3.1/VEGF165质粒植入坏死的股骨头内;第二组植入DPB;第三组仅在股骨头内钻一隧道。股骨头标本术后3天

8和16周获取。RT-PCR检测VEGF165mRNA的表达

Western blot检测VEGF165蛋白的表达

组织形态学分析血管发生和股骨头修复。结果:成功构建pcDNA3.1/VEGF165;第一组VEGF165 mRNA和蛋白表达术后1周达到高峰

时间持续超过3周。血管形成术后2

4周增加

骨形成术后2

4和8周增加

与另两组相比差异呈显著性（P<0.01）。结论:VEGF165基因转染可促进局部血管的早期形成

DPB-VEGF复合物可增加骨形成。脱蛋白骨联合VEGF165基因治疗为骨坏死的修复提供了理论基础。

Abstract

Objective:To reconstruct pcDNA3.1/VEGF165 and combine it with deproteinized bone(DPB) to repair avascular necrosis of the femoral head (AVNFH).Methods:Clone plasmid pUC18/VEGF165 containing the whole cDNA sequence of VEGF165 was combined with pcDNA3.1(+) to reconstruct pcDNA3.1/VEGF165.Sixty-nine AVNFH model New Zealand adult rabbits with a mean weight of 2.8 kg were randomly divided into three groups.In group 1

DPB combined with the reconstructed plasmid pcDNA3.1/VEGF165 was implanted in the drilled channel of the necrotic femoral head.In group 2

only DPB was implanted.In group 3

only channel was drilled without DPB or plasmid implanted.Femoral head specimens were obtained 3 days

and 8 weeks after operation.The expression of vascular endothelial growth factor (VEGF) was examined by RT-PCR

Western blotting techniques.Angiogenesis and repair of the femoral head were observed by histological and histomorphometric analysis.Results:pcDNA3.1/VEGF165 was reconstructed successfully.In group 1

the expression of VEGF was detected in the femoral head implanted by DPB-VEGF compound.VEGF165 mRNA and protein detected went up to apex 1 week postoperatively and lasted more than 3 weeks.The femoral head implanted by DPB-VEGF compound showed a significant difference in vascularization 2 and 4 weeks after operation and a significant difference in bone formation 2

4 and 8 weeks after operation from those in other groups on histomorphometric analysis (P<0.01).Conclusion:Transfection of VEGF165 gene enhances local angiogenesis in early time and DPB-VEGF compound improves bone formation.Deproteinized bone combined with VEGF gene provides a potential method for treatment of osteonecrosis.

关键词

脱蛋白骨血管内皮生长因子基因治疗股骨头缺血坏死

Keywords

Deproteinized bonevascular endothelial growth factorgene therapyAvascular necrosis of the femoral head

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