The experimental study on phosphatidylinositol 3-kinase/Akt in neural stem cells in vitro

WANG Shuang; et al

doi:10.13406/j.cnki.cyxb.2005.04.002

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The experimental study on phosphatidylinositol 3-kinase/Akt in neural stem cells in vitro

更新时间：2024-01-17

- The experimental study on phosphatidylinositol 3-kinase/Akt in neural stem cells in vitro
- Journal of Chongqing Medical University Issue 4, Pages: 489-492(2005)
- 作者机构：
  
  1. 重庆医科大学附属第一医院神经内科
  2. 重庆医科大学附属第一医院普外科
  3. 重庆医科大学附属第一医院神经内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.04.002
  CLC： R329.2
- Published：2005，
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[1]王爽,贾延劼,谢鹏,牟君,赵裕光,王运良,董为伟.磷脂酰肌醇-3激酶/Akt在体外神经干细胞存活和分化中作用的实验研究[J].重庆医科大学学报,2005(04):489-492+496.

WANG Shuang, et al. The experimental study on phosphatidylinositol 3-kinase/Akt in neural stem cells in vitro. [J]. Journal of Chongqing Medical University (4):489-492(2005)
[1]王爽,贾延劼,谢鹏,牟君,赵裕光,王运良,董为伟.磷脂酰肌醇-3激酶/Akt在体外神经干细胞存活和分化中作用的实验研究[J].重庆医科大学学报,2005(04):489-492+496. DOI： 10.13406/j.cnki.cyxb.2005.04.002.

WANG Shuang, et al. The experimental study on phosphatidylinositol 3-kinase/Akt in neural stem cells in vitro. [J]. Journal of Chongqing Medical University (4):489-492(2005) DOI： 10.13406/j.cnki.cyxb.2005.04.002.

摘要

目的:探讨磷脂酰肌醇-3激酶/Akt信号途径在体外培养神经干细胞存活、分化中的作用。方法:分为对照组、wortmannin组和LY294002组;免疫组化检测Nestin、NSE和S100的表达;TUNEL评价细胞凋亡率;Western Blotting检测磷酸化Akt、Caspase-9的表达。结果:随着wortmannin和LY294002浓度增大

细胞凋亡率逐渐增加。随着wortmannin和LY294002浓度增大

磷酸化Akt的表达逐渐减弱。高浓度时磷酸化的Caspase-9表达减弱。结论:神经干细胞存活依赖于PI3K/Akt途径的活化

其机制可能是PI3K/Akt活化后

促进了Caspase-9等蛋白的磷酸化

抑制了细胞凋亡事件的发生。

Abstract

Objective:To discuss the function of PI3K (phosphatidylinositol-3 kinase)/Akt signal pathway in proliferation and differentiation of neural stem cells (NSCs).Methods:Control group

wortmannin group and LY294002 group were randomly assigned.The expression of Nestin

NSE and S100 were detected by immunohistochemistry.The ratio of cell apoptosis was estimated by TUNEL staining and the expressions of phosphorylated Akt and Caspase-9 were detected by Western blotting.Results:With the increase in concentration of wortmannin and LY294002

the ratio of NSCs apoptosis increased.The results of Western blotting indicated that the expression of phosphorylated Akt decreased when the concentration of wortmannin and LY294002 increased.High concentration of wortmannin and LY294002 inhitited Akt phosphorylated.At the same time

the expression of phosphorylated Caspase-9 decreased.Conclusion:The survival of NSCs depends on the activation of PI3K/Akt pathway

but PI3K/Akt pathway may have little function in the differentiation of NSCs.

关键词

磷脂酰肌醇-3激酶（PI3K）Akt神经干细胞凋亡

Keywords

Phosphatidylinositol 3-kinase (PI3K)Aktneural stem cellsapoptosis

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