The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro

WANG Liang; et al

doi:10.13406/j.cnki.cyxb.2005.03.037

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The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro

更新时间：2024-01-17

- The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro
- Journal of Chongqing Medical University Issue 3, Pages: 442-443(2005)
- 作者机构：
  
  1. 重庆医科大学临床学院普外科
  2. 重庆医科大学临床学院普外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2005.03.037
  CLC： R737.9
- Published：2005，
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[1]王良,任国胜,涂刚.外源性透明质酸酶对人乳癌细胞株体外增殖的影响[J].重庆医科大学学报,2005(03):442-443.

WANG Liang, et al. The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro. [J]. Journal of Chongqing Medical University (3):442-443(2005)
[1]王良,任国胜,涂刚.外源性透明质酸酶对人乳癌细胞株体外增殖的影响[J].重庆医科大学学报,2005(03):442-443. DOI： 10.13406/j.cnki.cyxb.2005.03.037.

WANG Liang, et al. The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro. [J]. Journal of Chongqing Medical University (3):442-443(2005) DOI： 10.13406/j.cnki.cyxb.2005.03.037.

摘要

目的:探讨外源性透明质酸酶（Hyaluronidase

Hyase）对人乳癌细胞株增殖的影响。方法:应用MTT法和流式细胞仪观察外源性Hyase对人乳癌细胞株增殖率及细胞周期分布的影响。结果:ZR-75-30细胞株实验组A值（0.674±0.221）明显高于对照组（0.563±0.046）

P<0.05;实验组S期细胞百分率（%）为18.36±0.65

对照组为2.19±0.10;实验组G0/G1期细胞百分率（%）为44.49±4.33

对照组为62.14±26.97

两组比较均有显著性差异（P<0.05）。MDA-MB-435细胞株实验组A值（0.694±0.054）明显高于对照组（0.578±0.072）

P<0.05。MDA-MB-231细胞株实验组A值（0.736±0.059）明显高于对照组（0.637±0.046）

P<0.05。结论:外源性Hyase可能具有促进人乳癌细胞株增殖的作用。

Abstract

Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674±0.221) was significantly higher than that in control group(0.563±0.046).The percentage of phase S cells in study group(18.36±0.65) was higher than that in control group(2.19±0.10);the percentage of phase G0/G1 cells in study group(44.49±4.33) was lower than that in control group(62.14±26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674±0.221) was significantly higher than that in control group(0.563±0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674±0.221) was significantly higher than that in control group(0.563±0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

关键词

透明质酸酶乳癌细胞周期

Keywords

HyaluronidaseBreast cancercell cycle

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